1 AIT Asian Institute of Technology

Extraction of leaf protein concentrate from water hyacinth

AuthorHu, Xiaopei
Call NumberAIT Thesis no.AE-00-01
Subject(s)Water hyacinth

NoteA thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering, School of Environment, Resources and Development
PublisherAsian Institute of Technology
Series StatementThesis ; no. AE-00-01
AbstractWater hyacinth juice was extracted by extruding water hyacinth through a screw press. Leaf protein concentrate was collected by centrifuging the juice after heat and acid treatment. Electric heater, water bath, steam, and microwave were used for testing the thermal effects on the quantity as well as the quality of extracted leaf protein coagulum. Hydrochloric acid, formic acid, lactic acid and fermented brown deproteinized juice were used in an attempt to find the acidified conditions for getting maximum quantity and better biological and functional values of leaf protein concentrate, Combined heat and acid coagulation method was also explored under selected thermal and acid conditions. Protein content was the indicator of the quantity of extracted leaf protein concentrate while total lipid, solubility and digestibility were considered ad quality factors. Proper extraction technology was selected by comparing different thermal and acid coagulation methods. Maximum protein recovery rate appeared for water bath treatment at temperature of 50'c for 20 min duration and by adjusting the pH of water hyacinth juice to 4.5 by adding 10% formic acid. Under this condition, 2.3 g pure protein can be obtained from 1000 g fresh water hyacinth and the protein extractability was about 34.4%. The highest solubility and lowest crude fat content of 12.1% and 1.73% respectively were obtained under the coagulation methods of 40'c water bath treatment for 15 min duration and 1.35 atm steam injection for 4.5 min. High protein digestibility of 55.5% was obtained for combined formic acid and 50'c heating method. Considering both the biological as well as the functional properties of leaf protein concentrate, optimum fractionation technology was selected by using combined formic acid and heat treatment. Encouraging potential was indicated for utilization of leaf protein concentrate from water hyacinth as s promising protein source.
Year2000
Corresponding Series Added EntryAsian Institute of Technology. Thesis ; no. AE-00-01
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSAgricultural and Food Engineering (AE)
Chairperson(s)Salokhe, V.M.;
Examination Committee(s)Singh, Gajendra;Rakshit, Sudip Kumar;Chaiyaphol Kaewprakaisaengkul;
Scholarship Donor(s)H. M. the King of Thailand;
DegreeThesis (M.Eng.) - Asian Institute of Technology


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