1 AIT Asian Institute of Technology

Effect of controlled and modified atmosphere storage on qualities and senescence of Dendrobium orchid

AuthorWarinthon Yimyong
Call NumberAIT Diss. no.AE-14-02
Subject(s)Protective atmospheres
Orchids--Storage

NoteA dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Engineering in Agricultural S ystems and Engineering
PublisherAsian Institute of Technology
AbstractThis research work deals with the effects of controlled atmosphe re storage and modified atmosphere packaging (MAP) on the quality and senescence of cut Dendrobium orchid flowers. The controlled atmospheres were combinations of 2, 4, 6 and 8% O 2 and 5 and 10% CO 2 , thus resulting in a total of eight different controlled atmospheres, while the normal atmosphere storage was of 21% O 2 and 0.03% CO 2 . All treatments were retained i n low temperature storage of 13  C and 95 % relative humidity (RH). The results show that cut Dendrobium orchids stored in the 2% O 2 and 5% CO 2 cont rolled atmosphere had the longest storage life of 28.33 days, whereas cut Dendrobium orchids in the normal atmosphere possessed the shortest storage life of merely 11.67 days. Furthermore, after 28.33 days the orchid flowers in the 2% O 2 and 5% CO 2 atmosph ere could significantly retain more fresh weight, anthocyanin content and freshness than cut Dendrobium orchids in the normal atmosphere storage. This dissertation experimented with 10 MAP treatments, consisting of five treatments of orchid flowers wrapped with polyethylene (PE), polypropylene (PP) , low density polyethylene (LDPE), high density polyethylene (HDPE) and polyvinyl chloride (PVC) films and another five treatments wrapped with the same plastic film types and filled with the controlled atmosphere of 2% O 2 and 5% CO 2 . All ten treatments were completely sealed prior to storage at 13 o C and 95% RH. The results indicate that orchid flowers packed inside the MAPs of PE film, LDPE film, HDPE film, PVC film and PP film had their respective average storage life of 12, 15, 15, 12 and 18 days. However, the orchid flowers packed inside the MAPs filled with the optimum controlled atmosphere of 2% O 2 and 5% CO 2 had average storage life of 15 days, whereas the flowers retained in the normal atmosphere condition l asted for merely 9 days . Moreover, all MAPs were found to lower weight loss, respiration rate, ethylene production and anthocyanin content of orchid flowers. The controlled atmosphere storage (CA) of 2% O 2 and 5% CO 2 together with the polypropylene film (P P) MAP acted positively in delaying aging and biochemical changes involved in petal senescence of Dendrobium orchid ̳Queen Pink‘. The delayed aging and senescence are attributable to the decreasing rates of ACC synthase and ACC oxidation activities, to del ayed degradation of total protein, to decreasing rate of protease activity , and to the reduced electrolyte leakage from the cells as the leakage is responsible for loss of turgor and visible wilting.
Year2014
TypeDissertation
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSAgricultural and Food Engineering (AE)
Chairperson(s)Soni, Peeyush;
Examination Committee(s)Athapol Noomhorm;Oanh, Nguyen Thi Kim;Danai Boonyakiat;
Scholarship Donor(s)Rajamangala University of Technology Thanyaburi (RMUTT), Thailand;
DegreeThesis (Ph.D.) - Asian Institute of Technology, 2014


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