Design, simulation and experimental study of a droplet-based PCR by electrowetting on dielectric | |
| Author | Kessararat Ugsornrat |
| Call Number | AIT Diss. no.ME-10-01 |
| Subject(s) | Polymerase chain reaction Dielectrics |
| Note | A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Microelectronics, School of Engineering and Technology |
| Publisher | Asian Institute of Technology |
| Series Statement | Dissertation ; no. ME-10-01 |
| Abstract | In this thesis, a droplet-based polymerase chain reaction (PCR) with electrowetting on dielectric (EWOD) is developed to focus on minimizing reagent consumption without the use of a mechanical mechanism to move the sample in the system. The droplet-based PCR system consists of a parallel-plate EWOD mi crochannel and serpentine-shape microheaters integrated underneath to ma intain temperature for denatu ration, annealing, and extension. Silicone oil is introduced at the bottom of the microchannel to reduce temperature impact from control electrodes, prevent evaporati on and increase the cont act angle reduction of droplets. The EWOD actuation is used to cont rol the droplet in a PCR system. The EWOD device is fabricated and tested for droplet moving. EWOD configur ation and serpentine- shape microheaters are designed based on theore tical calculation and finite element (FEM) simulation. The simulation results indicate that the applied voltage of control electrodes does not affect the temperatur e of the microchannel. In ad dition, the optimized applied voltages of three microheaters for the te mperature of PCR are 1.08, 0.57 and 0.91 V, respectively. The contact angle characteriza tion shows a significant increase in contact angle reduction by silicone oil application. More over, the use of silicone oil allows higher applied voltage for transporting a droplet without evaporation. The thermal characterization of PCR microchannel show that the temperature of microheaters is in good agreement with simulation. |
| Year | 2010 |
| Corresponding Series Added Entry | Asian Institute of Technology. Dissertation ; no. ME-10-01 |
| Type | Dissertation |
| School | School of Engineering and Technology (SET) |
| Department | Department of Industrial Systems Engineering (DISE) |
| Academic Program/FoS | Microelectronics (ME) |
| Chairperson(s) | Afzulpurkar, Nitin; |
| Examination Committee(s) | Adisorn Tuantranont ;Lertsak Lekawat; |
| Scholarship Donor(s) | King Mongkut’s University of Technology North Bangkok, Thailand; |
| Degree | Thesis (Ph.D.) - Asian Institute of Technology, 2010 |