1 AIT Asian Institute of Technology

Antibiogram analysis of isolated E.coli and salmonella from beef meat.

AuthorKalhoro, Muhammad Saleem
Call NumberAIT RSPR no.FB-15-01
Subject(s)Salmonella
Foodborne diseases--Thailand
Antibiotics
Pathogenic bacteria

NoteSubmitted in partial fulfilment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
PublisherAsian Institute of Technology
Series StatementRSPR ; no. no.FB-15-01
AbstractThis research study was conducted to determine the occurrence of E.coliand Salmonellain beef and to study their antibiotic resistance profiles. Beef samples (15) were collected fromTalad Thaimarket, Pathum Thani area, Thailand and analyzed through culture methods for possible contamination of E. coliand Salmonella.E. coliwas found in 9 out of 15 samples (60%), while Salmonella was found in 3 samples (20%). All 12 isolates were confirmed through biochemical tests which included: TSI, Indole test, Methyl red test, Voges Proskauer test, and Citrate test. Antibiogram analysis revealed that bacterial isolates were resistant against some tested antibiotics.E.coliresistance was recorded against Ampicillin, Amoxicillin and Tetracycline (44.44%), Sulfamethoxazole/Trimethoprim, Chloramphenicol (22.22%) and Streptomycin (11.11%). All Salmonellaisolates were resistant against Tetracycline (100%) and Chloramphenicol (33.33%).This study confirms the presence of E. coliand Salmonellain beef meat and their resistance towards common commercial antibiotics which may be potential threat to consumer’s health. Good hygienic practices during the slaughter processing, transport and storage of beef can reduce the contamination.
Year2015
Corresponding Series Added EntryAsian Institute of Technology. RSPR ; no. no.FB-15-01
TypeResearch Study Project Report (RSPR)
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSFood Engineering and Bioprocess Technology (FB)
Chairperson(s)Loc Thai Nguyen;Anil Kumar Anal;
Examination Committee(s)Athapol Noomhorm;Datta, Avishek;
Scholarship Donor(s)Higher Education Commission (HEC), Pakistan-Asian Institute of Technology Fellowship;
DegreeResearch Studies Project Report (M. Eng.) - Asian Institute of Technology,2015


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