Triploidy induction and growth performance of hybrid catfish (Clarias macrocephalus x C. gariepinus) | |
| Author | Parven, Afshana |
| Call Number | AIT Thesis no.AQ-07-27 |
| Subject(s) | Catfishes Hybridization Hybridization |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-07-27 |
| Abstract | Cold shock and heat shock were evaluated for their efficacy as methods of manipulating ploidy in hybrid catfish (C. macrocephalus x C. gariepinus). The most effective method of inducing triploidy was cold shock at 6°C applied of 25 min which resulted in 87% triploidy with 48% fry survival rate from fertilization to two weeks rearing period. Both of thermal shock treatment initiated 3 minutes after water activation. Cold shock treatment for 30 min or more caused total mortality of embryos. Heat shock at 40°C for 1 min resulted 42% triploidy with fry survival of 42%. Flow cytometry analysis was used to assess the ploidy level of diploid and triploid larvae in both experiments with blood samples. In growth experiment, growth of triploid was higher than control but lower than the cold shocked (6°C 25 min) diploid fry, with the survival rate ranging between 63-73% in all treatments after 16 weeks of rearing period. Triploid fish were 15% larger than control; while shocked diploid was 27% larger than control. |
| Year | 2007 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-07-27 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Gallardo, Wenresti G.;Bart, Amrit N. |
| Examination Committee(s) | Yakupitiyage, Amararatne;Yi, Yang |
| Scholarship Donor(s) | Norway;AIT Fellowship |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2007 |