| Author | Rungkarn Suebsing |
| Call Number | AIT Thesis no.AQ-09-11 |
| Subject(s) | Macrobrachium rosenbergii--Diseases
|
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-09-11 |
| Abstract | Macrobrachium rosenbergii is one of the most important and economically farmed prawn species in Southeast Asia, including Thailand. Among them, white tail disease (WTD) is the major constraint caused by Macrobrachium rosenbergii nodavirus (MrNV). Therefore, there is a need to develop a diagnosis method for MrNV infection in giant freshwater prawn farms. In this study, the sensitivity and cost effectiveness between one-tube and two-tube reverse transcriptase (RT) nested PCR by amplifying ten-fold series of total RNA were compared. The results demonstrated one-tube technique had the highest sensitivity (≥ 0.1 fg of total RNA), specificity and lowest cost (354.77 THB) than two-tube method (1 fg of total RNA; 362.76 THB). In addition, one-tube technique was preferred to measure the development of new RT nested-PCR in the field samples. A total of 133 samples were randomly collected from hatcheries and ponds in central Thailand. Sixty out of 133 samples (45.11 %) were MrNV positive. Also, a total of 29 wild prawns collected from stocked prawns in Pak-Mun Dam and Tapee River revealed that 4 wild samples were MrNV infections indicating it might infect with the same MrNV from hatcheries due to waste water were directly discharged from several farms to natural rivers. Moreover, we identified 3 silent positions and 1 mutation position resulting in a change of RNA-2 deduces amino acid at position 169 from alanine to threonine. This study provided the evidence that MrNV disease outbreaks occurred in farmed prawn in central Thailand and in natural resources and this is a first reported of the MrNV incidence in Thailand. The information obtained from this study will lay the dissemination for farm management and quarantine method development to farmers. |
| Year | 2009 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-09-11 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Yakupitiyage, Amararatne;Rakshit, Sudip |
| Examination Committee(s) | Gallardo, Wenresti G. |
| Scholarship Donor(s) | AIT Fellowship;RTG Fellowship |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2009 |