A feasibility study of small scale soy protein concentrates (SPC) production and its use as a feed ingredient for nile tilapia (Oreochromis niloticus) juveniles | |
| Author | Marwa, Nyakorema Beatrice |
| Call Number | AIT Thesis no.AQ-10-11 |
| Subject(s) | Soy proteins Nile tilapia--Feeding and feeds Fishes--Feeding and feeds |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-10-11 |
| Abstract | This research was conducted to determine a suitable technique of extracting Soy protein concentrate (SPC) from defatted soy bean (DSBM) and evaluate the feeding value for Nile tilapia ( Oreochromis niloticus). A 2x3 factorial design was used to determine a suitable method for protein extraction. SPC was extracted by using two extraction methods (acid and alcohol wa sh) separated through three different mesh sizes (250, 125, and 38μm) as filtration materials and the products were subjected to two drying methods (sun and oven drying). It was found that acid wash filtration through 125 and 38μm mesh sizes and sun drying produced a product with high protein content, whereby prot ein from both mesh sizes were not significantly different (60.1 and 60.7% P<0.05). The product had a protei n recovery rate of 23% (ranging from 49.2 to 60.7%) and solid recovery rate of 63%. Therefore, acid wash filtration through 125 μm mesh size and sun drying was considered as a simple extraction method in producing SPC for small scale production. A feeding trial was conducted in a completely randomized design to evaluate the effect of extracted SPC as a major protein source on the growth performance of Nile tilapia ( Oreochromis niloticus) fed experimental diets made fr om the product and control diets. Seven diets were formulated c ontaining 30% protein. Fish meal inclusion levels in the SPC dietary diets were 0, 3, 6 and 10% protein ratio, where as fish meal inclusion level of 0% protein ratio was supplemented with methi onine. Two control diet s included fishmeal (12%) and soybean meal which excluded fishmeal . Each dietary treatment was tested in 3 replicates. Twenty (20) Nile tilapia juveniles of an individual weight of 5.8±0.19 g were stocked in 1x1 m 3 hapas immersed in outdoor non recirculation tanks (5m 3 ). Fish were fed twice a day at 08:30 and 14:00 hours, at 6% of body weight for a period of eight weeks. The results showed that there were significan t differences (P<0.05) in growth parameters between fish fed control diet s (35.5 &35.01g) and experimental treatments (37.2 to 51.8g). A similar trend was observed in apparent net protein utilization; i.e control diets had 21.6 & 22% () while experimental diets had a ra nge of 29.2 to 31.4%. Growth performance of fish fed diet with fish meal inclusion level of 0% of protein ration in a SPC dietary diet with methionine (42.7g) supplementation was si milar to those fed a diet of fishmeal inclusion level of 3% of protein ration in SPC dietary diet in (48.57g). In conclusion, diet with fishmeal inclusion level of 0% with methionie was preferred for Nile tilapia ( Oreochromis niloticus) juvenile reared in outdoor non- recirculated tanks followed by fishmeal inclusion levels of 3% pr otein ratio in SPC dietary diet. The results of this work indicate that SPC can be extracted simply by using acid wash filtrate through 125 meshes size and sundrying. SPC is proved to be useful in aqua feed and is capable of being used as a complete practical diet for the growth of Nile tilapia( Orechromis nailoticus ) juveniles. |
| Year | 2010 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-10-11 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Amararatne Yakupitiyage; |
| Examination Committee(s) | Gallardo, Wenresti;Anal, Anil Kumar; |
| Scholarship Donor(s) | Ministry of Livestock Development and Fisheries, Tanzania; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2010 |