Use of AIT cafeteria wastewater and pond water as alternative media for the culture of the cyanobacteria Nostoc paludosum and conditions for optimizing biomass and lipid production | |
| Author | Mellisa, Siska |
| Call Number | AIT Thesis no.AQ-13-03 |
| Subject(s) | Sewage--Purification--Nutrient removal Lipids Sewage--Purification--Biological treatment |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resource Management, School of Environment, Resources Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-13-03 |
| Abstract | The study was conducted to identify the possibility of using AIT cafeteria wastewater and pond water as an alternative media for culturing Nostoc paludosum. However, cafeteria wastewater proved to be inappropriate, probably as a result of changing composition. Wastewater collected during successive days led to massive algal death on most culture, but also to survival on some days. Enrichment of wastewater by adding urea and triple super phosphate did not change the result and N. paludosum did not survive. Alternative growing technologies were investigated. Pond water can be used although it can produce contamination by Synechococcus sp., Gleocapsa sp., and Paramecium sp. However, different spikes of algae inoculation permit to minimize the contamination and increase the dominance of N. paludosum. Treatment using highest spikes of algae inoculation (20%) showed the best result. Next experiment was aimed at identifying the limiting nutrients of N. paludosum by using the bioassay method and urea as nitrogen source, K2HPO4 as phosphorous source and CaCO3 as carbon source. Nitrogen is the limiting nutrient. Next study was to identify the optimal frequency of nitrogen fertilization for N. paludosum. The result did not show difference when using different frequency of fertilization. N. paludosum aggregates in cultures creating difficulties for sampling and measuring biomass, as well as affecting algal growth. An experiment was set up to investigate if N. paludosum culture improves by using aeration and shaker. Biomass production was higher when cultured using aeration, this can be the result of improvement in the homogeneity of the algal solution, but also a higher availability of carbon dioxide for algal growth. Lipid content of N. paludosum using BG II media was investigated. The highest lipid content (7% of dry weight) was obtained after 11 days, when nitrate concentration is low (451 ppm). |
| Keyword | Nostoc paludosum; cafeteria wastewater; pond water; contamination; nutrients; lipid content |
| Year | 2013 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-13-03 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Dabbadie, Lionel; |
| Examination Committee(s) | Yakupitiyage, Amararatne Gallardo, Wenresti ; |
| Scholarship Donor(s) | The Government of The Province of Aceh, Indonesia ; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2013 |