Culture of microcystis aeruginosa in a chicken manure feritlized culture system for biodiesel production | |
| Author | Suntree Pumpuang |
| Call Number | AIT Thesis no.AQ-13-04 |
| Subject(s) | Biodiesel fuels Chickens--Manure |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-13-04 |
| Abstract | Cyanobacteria hav e significant potential to be a s ource to produce biodiesel . However, there are two major problems , namely the cost of nutrient sources and harvesting method. This study was conducted to determine the optimum dose of chicken manure as a cheap raw material to culture Microcystis aeruginosa , the optimum time to harvest M.aeruginosa, the effect of floccul ants on biomass, lipid yield and biodiesel production, evaluating the potential reuse of used nutrient solution for algae culture and the cost - effectiveness of flocculants. M.aeruginosa was cultured at dose of 3, 6, 9 and 12 g/L chicken manure. The chicke n manure dose of 6 g/L gave a higher cell density and specific grow rate than the other. The culture of M. aeruginosa was suitable for harvesting on the 5 th day of culture because they could provide higher lipid yield of 1.29% . The efficiency of the floccu lation method s by using alum without neutralization, alum with neutralization, sodium hydroxide without neutralization and sodium hydroxide with neutralization on the biomass, lipid yield and biodiesel production were studied. A lum without neutralization yielded highest d ry weight of biomass, However, the percentage of lipid production was highest with sodium hydroxide with n eutralization at 10.25 % and biodiesel production was highest with sodium hydroxide with out n eutralization an d a lum with neutralization at 1.31 mL /g of lipid , respectively. There was no significant difference among sodium hydroxide with neutralization, alum without neutralization and alum with neutralization. The water from flocculation was used to culture Microcystis aeruginosa for 14 days, the results showed the water from sodium hydroxide with neutralization gave higher cell density of 3.48 x 10 7 cells/ml. There was no significant difference among autoflocculation, NaOH with neutralization, alum without n eutralization and alum with neutralization except control and NaOH without neutralization . The specific growth rate of M. aeruginosa was found higher in an alum without neutralizer of 0.28 day - 1 . There was no significant difference among control, autoflocc ulation, NaOH with neutralization, alum without neutralization and alum with neutralization except NaOH without neutralization. The cost - efficiency of the flocculation method to harvest the algae cell is alum without neutralization because it gave high yie ld of biomass when compared with other flocculants, which cost about 10 baht per ton of algae culture. These results are expected to be useful for further studies and in improving the techniques to produce cyanobacteria for biodiesel production. |
| Year | 2013 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-13-04 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Yakupitiyage, Amararatne |
| Examination Committee(s) | Gallardo, Wenresti G.;Dabbadie, Lionel |
| Scholarship Donor(s) | RTG Fellowship;AIT Fellowship |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2013 |