Potential for culturing Nostoc muscorum and Synechococcus elongatus in tofu wastewater as a biodiesel feedstock sources | |
| Author | Rusydi, Rachmawati |
| Call Number | AIT Thesis no.AQ-13-05 |
| Subject(s) | Tofu Feedstock Sewage--Biological treatment |
| Note | A thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-13-05 |
| Abstract | This study was conducted to evaluate potential for culturing Nostoc muscorum and Synechococcus elongatus as a biodiesel feedstock source in tofu wastewater. An evaluation of the growth rate and biomass production of Nostoc muscorum and Synechococcus elongatus in BG - II medium and tofu wastewater were evaluated. Nostoc muscorum, multicellular Cyanobacteria, grew well in BG - II medium at a rate of 0.10 μg day - 1, while Synechococcus elongatus, unicellular Cyanobacteria, grew at arate of 0.24 μg day - 1. Chlorophyll - a content of Nostoc muscorum and Synechococcus elongatus were increased by 916.5% and 346.9% respectively. Lipid content of Nostoc muscorum was slightly higher than Synechococcus elongatus cultured in BG - II medium. The nutrient requirement of Nostoc muscorum and Synechococcus elongatus was determined by culturing tham in different N and P concentration of BG - II medium. It was found that Nostoc muscorum required 644.68 mg/L of NO 3 - and 25.16 mg/L of HPO 4 - and the high lipid production was gained on day 4 of culture. Synechococcus elongatus cultured in BG - II medium required 289.11 mg/L of NO 3 - and 22.26 mg/L of HPO 4 - and the high lipid production was obtained on day 6 of culture. Tofu wastewater consisted of soybean immersion waste, soybean washing waste, and whey with at a ratio of 1:2:0.5, respectively. Nostoc muscorum grew well when tofu wastewater was dialuted at a ratio of 40: 60% with distilled water. The nutrients required for supporting good growth was 128.94 mg/L of NO 3 - and 23.63 mg/L of HPO 4 - , while the high lipid production was gained on day 7 of culture. For Synechococcus elongatus gained fair growth at 20% of tofu wastewater. The nutrients concentration for supporting the growth was 386.81 mg/L of NO 3 - and 20.82 mg/L of HPO 4 - . The highest lipid production was gained on day 6 of culture. Lipid content of Nostoc muscorum was higher than that of Synechococcus elongatus cultured in tofu wastewater Identification of the appropriate pre treatment method for disrupting cells of Nostoc muscorum and Synechococcus elongatus for fatty acids extraction was evaluated using five methods, namely: homogenization, ultrasonication, thermolysis, osmotic shock, and acid - heat treatment. Nostoc muscorum cells were effectively disrupted by acid - heat pretreatment and lipid yield was 10 times higher than untreated Nostoc muscorum. Synechococcus elongatus did not respond well to pretreatments. For eample, osmotic shock resulted in lipid content almost similar with untreated Synechococcus elongatus. Based on the result, it can be concluded that Nostoc muscorum has the potential for using biodiesel feedstock by using tofu wastewater as its medium of culture. Aid - heat pretreatment is an effective method for dirupting Nostoc muscorum cells for lipid extraction. |
| Year | 2013 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-13-05 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Yakupitiyage, Amararatne |
| Examination Committee(s) | Gallardo, Wenresti;Dabbadie, Lionel;Anal, Anil Kumar |
| Scholarship Donor(s) | The Government of the Province of Aceh, Indonesia |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2013 |