Fermented chicken feather as an alternative protein source for Nile Tilapia (Oreochromis niloticus) | |
| Author | Lumpan Poolsawat |
| Call Number | AIT Thesis no.AQ-15-01 |
| Subject(s) | Nile Tilapia Aquaculture Fishes--Feeding and feeds Nile Tilapia--Feeding and feeds |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aqu aculture and Aquatic Resources Management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-15-01 |
| Abstract | Three laboratory - scale experiments were conducted to evaluate the potential for converting chicken father into an alternative protein source for fish by fermenting it with Bacillus spp. The first experiment was c onducted to evaluate keratinolytic activity of Bacillus licheniformis , Bacillus megaterium and Bacillus subtilis . In the second experiment, optimal bacterial inoculation rate , pH , te mperature and fermentation time were assessed. The third experiment was co nducted to assess the proximate composition and amino acid profile, and apparent dry matter and protein digestibility of fermented feather meal. All experimental trials had 3 replicates. The first experiment had six treatments: inoculation of chicken feat her with Bacillus subtilis , Bacillus megaterium and Bacillus licheniformis stains obtained from Thai Department of Fisheries (DOF), and the same strains obtained from Thailand Institute of Scientific and Technological Research ( TISTR) i.e. Bacillus subtili s TISTR 1248 , Bacillus subtilis TISTR1415, and Bacillus licheniformis TISTR1904. The result showed Bacillus subtilis DOF strain had the significantly higher (p ≤ 0.05) keratinolytic activity. There were four experimental trials in the second experiment: (1) bacterial inoculations at the rates of 1.5, 3.0, 4.5, 6.0 and 7.5×10 6 CFU /ml; (2) three pH in chicken feather med ia ( 5, 7 and 9); two incubation temperatures (room temperature and 37 o C); and (4) five fermentation time (1, 2, 3, 4 and 5 days) . Optimal fermentation conditions for on keratinolytic activity were found to be 4.5 ×10 6 CFU /ml of bacterial inoculation, neutra l incubation pH (7), 37 o C of incubation temperature, and 3 days of fermentation time. During the third experiment, proximate composition analyses of fermented feather meal showed it contained 81.7% crude protein, 2.3% crude lipids, 0.05% fiber and 10.9% a sh. Amino acid analyses showed lysine, methionine and histidine contents were 2.1, 0.75, and 0.77 g/100g protein, respectively. The chemical score showed first, second and third limiting factors of fermented feather meal protein for Nile tilapia were methi onine, lysine and histidine, respectively. The digestibility trial showed that protein digestibility is about 70%. Although fermented feather meal is a promising alternative protein source, further research are required to find ways to improve its amino a cid profile and digestibility. |
| Year | 2015 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-15-01 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Yakupitiyage, Amararatne |
| Examination Committee(s) | Salin, K.R.;Anal, Anil Kumar |
| Scholarship Donor(s) | Thailand (HM Queen) |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2015 |