| Abstract | Pyrazolidine Luminol (PL) was found as colorimetric chemosensor for Cu(II) ion. Upon addition of Cu(II) ion, PL sensor induced a color change from colorless to yellowish green. Hence, development of PL sensor to become PL strips was needed to be investigated. Preferable of PL sensor to metals ion was considered by held selectivity test. Detection limit was found by notice the lowest color produced of PL that can be seen by naked eye upon addition Cu(II) ion solutions. Optimum condition of PL strips was found in order to make PL strips give high sensitivity. Not only observation by naked eye, but also UV-vis spectrophotometer were needed to conclude that PL sensor was sensing with Cu(II) ion. Examination of strips was held by comparing their results with ICP OES measurements. Selectivity test revealed that PL sensor was highly selective to Cu(II) ion compare with other ions such as Ni(II), Ag(I), Hg(II), Cd(II), Pb(II), Zn(II), Mn(II), Co(II), Na(I), Fe(II), Ba(II), Al(II), Ca(II). UV-vis spectrophotometer measurement show that appearing new peak at 425 nm of PL wavelength was indication of PL as Cu(II) ion chemosensor. Highest interference while sensing Cu(II) ion was belong to Ag(I) and Cd(II) ions at 1000 equivalent. As increasing of concentration Cu(II) ion, the peak produced at 425 nm was shown increasing gradually. Moreover, detection limit of PL sensor was found at 3.75 x 10-4 M (23.8 mg/l) by naked eye observation and 0.6 x 10-4 M (3.8 mg/l) by UV-Vis spectrophotometer results. Calibration plot for detection Cu(II) ion was set up at concentration of Cu(II) ion 10-4 M to 10-3 M in high correlation of absorbance produces by PL sensor and concentration of Cu(II) ion (R2 = 0.9732). Linear working range of this plot was given by equation y = 0.3761x + 0.0203. Optimum condition to PL sensor in sensing Cu(II) ion was studied. Silica gel plate was chosen as best membrane that gives color change clearly than PTFE, Nylon, and filter paper. Dipping time of PL silica strips into Cu(II) ion brought optimum condition at 20 minutes. Influence of pH and volume of sample also studied. It was concluded that 10 ml of volume sample and neutral pH gave optimum condition to strips to produce color clearly. Those conditions make pH strips own detection limit as 0.16 x 10-3 M (10 mg/l). Examine of PL silica strips to synthetic wastewater at concentration 5, 25, 50, 100 and 500 mg/l offerred the results as non detection, 23.3± 5.8, 46.7± 11.5, 86.3± 23.1, and 433.3±57.7 μg/l, respectively. Cu(II) ion of water and wastewater sample from surface water, tap water, drinking water, laboratory wastewater and industrial (paint, fluorescent lamp and electroplating industries) wastewater were measured by ICP OES and PL silica strips. Since ICP OES results were range from 0.105 ± 0.010 to 1.127 ± 0.134 μ g/l, PL strips measurements were not conducted due to under detection limit of strips. |