Effects of arsenic exposure on inflammatory responses in human lymphoblast cell line and arsenic exposed children | |
| Author | Moh Moh Thant Zin |
| Call Number | AIT Thesis no.EV-13-12 |
| Subject(s) | Water--Purification--Arsenic removal Arsenic waste Lymphoblastoid cell lines |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. EV-13-12 |
| Abstract | Arsenic, a ubiquitous environmental pollutant, is a known human carcinogen. Exposure to arsenic has been associated with cancer and chronic diseases in human such as diabetes mellitus and ischemic heart diseases as a result of arsenic induced inflammation. A number of studies reported that prenatal arsenic exposure has been related to health risk of children. This study aimed to assess arsenic exposure and inflammatory responses in vitro and in arsenic-exposed-children. Arsenic-exposed-children from Ron Pibul sub-district and matched control children were recruited in this study. Arsenic exposure was determined by measurement of arsenic contamination in water (household drinking and non-drinking water), arsenic contamination in saliva (biomarker of arsenic exposure) as well as evaluates level of pro-inflammatory cytokines, IL-6 and IL-8, as a biomarker of inflammatory responses. In vitro determination of pro-inflammatory cytokines was performed using lymphoblast cell line. The study clearly demonstrated a dose-response relationship between level of arsenic treatment and pro-inflammatory gene expression in lymphoblast cell line which statistically significant was observed at 10μM. For human study, the level of arsenic in drinking water and non-drinking water consumed by arsenic-exposed-children were statistically significantly higher than control children by 2-fold (P< 0.01) and 10-fold, (P< 0.001) respectively. Salivary arsenic concentration in exposed-children was also statistically significantly higher than control children by 5-fold (P<0.05). The pro-inflammatory gene expression determined in saliva showed that IL-6 in exposed-children was 1.5-fold higher than the control children, while IL-8 gene expression was 1.8-fold statistically significantly (P<0.05) higher than the exposed-children. Although arsenic concentration in water was higher in exposed site, the mean arsenic level in the drinking water was in the acceptable range of WHO guideline (10 μg/L). The significant correlation between IL-6 and total arsenic concentration in children’s saliva suggested that arsenic-exposed-children may increase in risk of inflammatory diseases later in life. |
| Year | 2013 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Panida Navasumrit;Shipin, Oleg V.; |
| Examination Committee(s) | Jantamas Kanitwithayanun;Pornpat Intarasunanont; |
| Scholarship Donor(s) | Deutscher Akademischer Austausch Dienst (DAAD), Germany;Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2013 |