Isolation and characterization of Bacteroides bacteria as a tool for tracking sources of fecal pollution for animals | |
| Author | Yuranan Leknoi |
| Call Number | AIT Thesis no.EV-14-22 |
| Subject(s) | Bacteroides Water--Pollution |
| Note | A thesis submitted in partial fulfillment of the requirements for thedegreeof Master of Science in Environmental Engineering and Management Inter-University Program on Environmental Toxicology, Technology and Management |
| Publisher | Asian Institute of Technology |
| Abstract | Microbial source tracking (MST) methods has been developed for protection water quality for microorganism contamination in water resources. Less developed countries (LDCs) which is poor of water quality management has serious health impact from human pathogens. bacteriophages infecting Bacteroides method is one of simply method of library-independent and culture-dependent that has been studied in many countries. Discrimination of fecal contamination between human and animal is more challenging for bacteriophages infecting Bacteroides method. There are 2 reference Bacteroides host strains, which is HSP40 and RYC2056. Previous studies indicated HSP40 strain seems highly specific to human fecal contamination and RYC2056 strain detected bacteriophages with high concentration but cannot identify human and animal fecal polluted in waters. Bacterial host strain for tracking animal sources has been interesting in current. Targets of animals included goat, sheep, dairy cattle and swine. Initially test had been found isolated bacterial host strains, for swine has possible than other animals. SP25, which is strain of swine, had been shown preferment to detect bacteriophages from their source. Species identification by PCR and DNA sequencing were performed with SP25 strain. As the result, SP25 strain was identified as Bacteroides fragilis. Animal fecal sources, there were dairy cattle, swine and ruminants (goat and sheep) were specific test by SP25 strain. SP25 strain showed positive detection of swine by 50% (16 of 32), and it cannot detect other animals fecal sources. For human fecal contaminated waters such as hospital sewage, wastewater treatment plants, etc. SP25 strain was given negative detection of all (22 samples). Anyway, twenty-five samples of human polluted canals in Bangkok, SP25 strain has been detected one sample. In sum of SP25 strain, it is able to apply as a microbial sources tracking for swine fecal contaminated in waters in Thailand. In addition, negative detection of SP25 strain does not determine the absence of swine fecal contamination. HSP40 reference strain is high specific with human in this study. It detected bacteriophages for human fecal polluted waters but did not detect bacteriophages for any animals. As the results, it is similar result with previous researches. Another one, RYC2056 had been detected high concentration in human fecal contamination. Moreover, it is able to detect bacteriophages from swine and dairy cattle. Exactly the same with previous studies, RYC2056 strain cannot discriminate of origin fecal sources. HSP40 reference strain can apply to microbial source tracking for human fecal polluted waters and RYC2056 reference strain are supported to apply as a microbial source tracking of fecal contaminated waters in Thailand. |
| Year | 2014 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Kwanrawee Sirikanchana;Thammarat Koottatep; |
| Examination Committee(s) | Dubbs, James M.; |
| Scholarship Donor(s) | Royal Thai Government Fellowship;Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology - Chulabhorn Research Institute - Mahidol University, 2014 |