Determination of hydrogen peroxide and peroxidase enzyme in Scirpus validus plants in constructed wetlands feeding with wastewater containing acetaminophen | |
| Author | Vo Hoang Nhat Phong |
| Call Number | AIT Thesis no.EV-15-27 |
| Subject(s) | Constructed wetlands Sewage--Purification Sewage disposal plants |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Environmental Engineering and Management |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. EV-15-27 |
| Abstract | Pharmaceuticals and personal care products (PPCPs) have increasingly attracted attention since the past few years due to their universal consumption as well as indiscriminate discharge to the aquatic environment. The constructed wetland as well as the behavior of Reactive oxygen species (ROS), antioxidant enzyme system in plant species was examined in this research for phytoremediation alternatives. The results stated that the degradation factor in this research depended on Acetaminophen (ACT) concentration level. In 1 ppb ACT stress, the plant uptake mechanism dominated from 19 to 68%. The microbial degradation mechanism would be from 24 to 32%. The media adsorption was negligible. The by product transformation and other mechanism treated from 3 to 41%. In 100 ppb stress, the plant uptake took from 1 to 2 % only. The microbial and photolytic degradation dominated 53% while media adsorption got9%. The by-product transformation and other mechanisms received from 1 to 46%. In term time variances effect in ACT removal efficiencies, the ACT concentration reduced proportionally. However, the level of removal efficiency depended on the contribution of various degradation factors and their mutual interaction. The condition without sunlight had the highest removal efficiency in the effluent which higher than 90%. Nevertheless, it might have come from the by-product transformation. The latter was with sunlight and with media support removed from 60 to 70% of ACT. The lowest was without plant condition just could treat below 50% of ACT in the effluent. Regarding plant uptaking, the ability increased with time variances as well as level of ACT concentrations. Scirpus validus could uptake around 0.2 μg ACT/g FW at maximum level. Plant without sunlight condition performed ineffective uptake ability. In pilot model, the half-life degradation was 12.3 days due to the interaction of various components in real wastewater and high level of ACT concentration. In the aspect of ROS and antioxidant system behavior, the shoot generated more H2O2 than the root while the root generated more antioxidant enzymes than shoot, in reverse. The H2O2 and soluble PO seem to have more active respond in the root than ionical and covalent PO. In pilot model, the "breakthrough" point happened in day 25 implying the adaptation of system. The pilot model was efficient in removing some contaminants as 81.1% TSS, 94.8% NH4-N, 88.1%COD, 67.1% TP. Moreover, the ACT was removed significantly with beyond 99% even though spiked 10,000 ppb. The most possible disturbed substance to ACT removal efficiency was TSS; however, it could be negligible due to the hydrophobic property of ACT. The organic bulk and rhizosphere might have supported the microbial degradation and plant uptake ability in the constructed wetland. The high organic content of wastewater also encouraged the sorption of hydrophobic compound as ACT. |
| Year | 2015 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Thammarat Koottatep; |
| Examination Committee(s) | Chongrak Polprasert;Visvanathan, C. ;Atitaya Panuvatvanich; |
| Scholarship Donor(s) | Greater Mekong Subregion (GMS) Scholarship; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2015 |