1 AIT Asian Institute of Technology

Improving biogas production from spirulina platensis by using fungal pre-treatment and co-digestion with cassava pulp

AuthorRattanasri Khajeefa
Call NumberAIT Thesis no.EV-17-17
Subject(s)Cassava machinery
Digestion
spirulina

NoteA thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Environmental Engineering and Management
PublisherAsian Institute of Technology
AbstractSpirulina platensis is a blue green algae that was studied for biogas production because of good biomass which can lead to high biogas production, but S. platensis has thickness of cell wall which can lead to difficult biological attack. Therefore, fungal pre-treatment which is pre-treatment technique can be carried out for disruption because this process is low cost and energy. In this study is dived two experiment. The first experiment is pre-treatment of S. platensis by using fungal pre-treatment which was evaluated effects of fungal pre-treatment for the suitable condition. The last experiment is co-digestion with cassava pulp which pretreated S. platensis was mixed with cassava pulp by adjustment C/N ratio at 15, 20 and 25 through Biochemical methane potential (BMP) test. In the first experiment, commercial Trichoderma sp. product was used for pre-treatment of S. platensis by various concentration (1%, 5%, 10% and 20%)for evaluated the best concentration. From the result of the first experiment found that at 20% of Trichoderma sp. product is the suitable for increasing of Hydrolysis yield (26.07%), Soluble sugar (8.83 mg/ml) and sCOD(77%) within 4 hours. Moreover, morphology of S. platensis after pre-treatment was also investigated. In the last experiment, biogas production of pretreated S. platensis was combined with cassava pulp for balance C/N ratio and it was compared with untreated and pretreated S. platensis alone through BMP test within 35 days. From the result of this experiment, pretreated S. platensis had the increasing of biogas and methane yield which are 20.94% and 26.24%respectivelywhen it compared with untreated S. platensis. However, ratio at C/N15 increased biogas yield (12.18%) and methane yield (5.4%) when it compared with untreated S. platensis. Therefore, the highest biogas and methane yield for this experiment are pretreated S. platensis alone without co-digestion. However, the best ratio for co-digestion with cassava pulp are C/N 15 and it is more than untreated S. platensis.
Year2017
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC))
Academic Program/FoSEnvironmental Engineering (EV)
Chairperson(s)Annachhatre, Ajit P.;
Examination Committee(s)Shipin, Oleg ;Anal, Anil Kumar;
Scholarship Donor(s)Royal Thai Government ;Asian Institute of Technology Fellowship;
DegreeThesis (M.Sc.) - Asian Institute of Technology, 2017


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