E. coli inactivation by using streptomycin producing organisms | |
| Author | Thanarat Theppharak |
| Call Number | AIT Thesis no.EV-17-27 |
| Subject(s) | Pathogenic bacteria--Inactivation Pathogenic microorganisms |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Environmental Engineering and Management |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. EV-17-27 |
| Abstract | Currently, disinfection system are highly concerned to remove the pathogen in water and wastewater. This research aims to evaluated to the performance of streptomycin producing organism inhibit E. coli in black water. The black water were collected from the effluent of Double Ring Cess-To-Fit technology (2RCTF). 2RCTF is a wastewater treatment unit that can retrofit to existing cesspool, which is used to treat blackwater from toilet. However, there are no previous study about E.coli inactivation by using streptomycin producing organism in the past based on research published. Evaluation of E.coli inhibition by commercial streptomycin in varied organic loading rate were studied at 40, 80 and 120 ug/ml of streptomycin with 50, 100, and 150 mg COD/Lin order to inhibit E.coli in blackwater. Streptomyces griseus strain BCC 3280 were obtained from Thailand Bioresource Research Center, and were studied in various characteristic such as zone of inhibition against E.coli on nutrient agar. Streptomyces griseus strain BCC 3280 were studied in batch scale with E. coli strain 527 in order to see the effect of Sreptomtces griseus on survival amount of E. coli under selected time and various conditions. The S. griseus were also apply to use in lab-scale of continuous flow reactors treating blackwater with and varying the effect of sponge media on E. coli inactivation. In summary of the research studies, Streptomyce griseus strain BCC 3280 have ability to produce a zone of inhibition versus E.coli strain 527under optimal growth conditions. Completed E. coli inactivation of commercial streptomycin in batch scale were achieved mostly at 24 hours. In batch test of S. griseus culture, they have capable to inhibit about 1 log reduction of E.coli in actual wastewater under optimal growth condition. For the factor affecting of E.coli inhibition by streptomycin producing organism are; dissolve oxygen, pH, temperature, volume ratio between S. griseus and others microorganism in the water, growth rate of S. griseus, streptomycin concentration and organic loading which were also discussed. |
| Year | 2017 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Thammarat Koottatep; |
| Examination Committee(s) | Visvanathan, C.;Nawatch Surinkul; |
| Scholarship Donor(s) | Royal Thai Government;Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2017 |