Multiplex PCR for rapid detection some of seafood pathogens | |
| Author | Tran Thi Cue Phuong |
| Call Number | AIT Thesis no.FB-08-08 |
| Subject(s) | Polymerase chain reaction DNA polymerases |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-08-08 |
| Abstract | Multiplex Polymerase Chain Reaction can playa major role in detection of seafood borne pathogens. This study describes the development of MPCR . system for the stimultaneous detection of Salmonella enteric a, Listeria monocygenes and Vibrio parahaemolyticus that are found in seafood. MPCR amplification of inv A gene of Salmonella enterica, hly gene of Listeria monocygenes, gyrB genes for Vibrio parahaemolyticus were used for detection. Four DNA extraction methods were studied and Triton X 100 method was found to be the best for all three indicator microorganisms. For simultaneous detection of these bacterial a annealing temperature of 58°C, DNA primer of concentration 2.5 IlM and volume of 2.5 III gave the best result on optimization. The sensitivity detection show that the lower detection limits were 104 cells / ml was obtained; enrichment with TSBYE broth for 6 hours, the detection limit 100 cells/ml. Thus, the MPCR system developed would help to ensure rapid and accurate detection of Salmonella enterica, Listeria monocygenes and Vibrio parahaemolyticus |
| Year | 2008 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-08-08 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Jayasuriya, H.P.W.; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2008 |