Production of leaf protein concentrate from cassava leaves | |
| Author | Asamaporn Juttupornpong |
| Call Number | AIT Thesis no.FB-08-12 |
| Subject(s) | Leaf proteins Cassava |
| Note | A thesis sub mitted in par tial fulfillm ent of the req uirem ents f or the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-08-12 |
| Abstract | Protein extraction from cassava leaves usmg different technique namely spontaneous precipitate, acid, heat, combination of acid and heat precipitate were compared on yield and quality of protein. The drying of extracted protein to be powder by hot air drying, vacuum drying, and freeze drying were investigated. The ultrafiltration membrane was also used to separate the leaves protein and investigate the effect of pressure (1.3, 1.5, 1.7 kg/cm²) and temperature (30, 35, 40°C) on flux rate. The application of extracted product on rice gruel was conducted. Heat precipitate gave high value of all physiochemical properties among the other methods. The yield, L, a, a/b, protein content, carotene content and xanthophyll content were 11 .61% (wet wt.), 30.33, -2.63, -0.14, 46.42%, 630.84ppm and 161.01ppm, respectively. Ultrafiltration membrane, transmembrane pressure and temperature were affect on cumulative volume and permeate flux. Increased of pressure and temperature resulted in an increased in volume and permeate flux. Pressure of 1.7 kg/cm² gave the maximum permeate volume and flux which was two times more than 1.7 kg/cm². The temperature at 40°C gave the maximum flux rate at the constant pressure. The leaf protein concentrate of ultrafiltration was measured the color (L, a, band a!b) value, protein content, carotene content and xanthophyll content. The result was 28.59, -4.21, 20.89, -0.20, 43.74%, 607.63ppm and 143.81 ppm, respectively. Each drying method was significantly difference in the physiochemical properties except protein content. 0.2 g of dried leaf protein concentrate (DLPC) was added on rice gruel and the results showed that the protein, carotene, xanthaphyll content were increased. Rice gruel treated with DLPC had more green in color compared to control. The preference score of both products were not significantly difference at 95% confident level. |
| Year | 2008 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-08-12 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Athapol Noomhorm; |
| Examination Committee(s) | Rakshit, Sudip Kumar;Jayasuriya, H.P.W.; |
| Scholarship Donor(s) | RTG Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2008 |