Enzymatic delignification of lignocellulosic rice straw and water hyacinth for biofuel application | |
| Author | Blanca, Melanie C. |
| Call Number | AIT Thesis no.FB-09-02 |
| Subject(s) | Water hyacinth--Thailand Straw as fuel--Thailand Lignin--Thailand |
| Note | A thesis submitted in partial fulfillment of the re quirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-09-02 |
| Abstract | Bioethanol is a renewable source of energy which is presently mixed with gasoline and can replace the continuously depleting fossil fuels. Lignocellulose is one of the substrate considered for bioethanol production of which is abundant, available, low cost, and non-food. This substrate includes several processing steps which include pretreatment. Enzymatic pretreatment has the advantage of being environmental friendly, causes lesser inhibition on subsequent steps, has milder reaction, requires lesser energy and does not necessitates specialized equipment. Two substrates which are available in large quantities were utilized namely Thai rice straw and water hyacinth. The percent lignin contents of these biomass were determined by two-step acid hydrolysis. For delignifying the feedstocks, four methods were utilized which were acid, laccase, laccase followed by acid and acid followed by laccase pretreatments. Laccase was combined with acid treatments since laccase alone is not helping in the release of release of sugar during enzymatic hydrolysis. The methods of pretreatments were tested by reducing sugar yield from hydrolysis using cellulase enzyme and by extent of delignification in which lignin components oftreated biomass were analyzed. The resulting lignin compositions for rice straw was 18.62% and for water hyacinth was 14.45% both in extractive free basis. Considering the maximum yield of reducing sugar from enzymatic saccharification, 0.3750 g per g biomass were produced from rice straw treated with 0.20% acid followed by 20 U laccase per g dry mass. On the other hand, water hyacinth maximum sugar yield amounted to 0.2867 g per g biomass which resulted from 0.20% acid pretreatment. The highest degrees of delignification for rice straw and water hyacinth were achieved using laccase enzyme pretreatment which resulted also to more available holocellulose in the treated biomass |
| Year | 2009 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-09-02 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Yakupitiyage, Amararatne; |
| Scholarship Donor(s) | Korea; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2009 |