Studies on phytochemical screening and biological activities of Thai herbal formulations Triphala and Solot Benjakul | |
| Author | Chonthicha Thanathitipong |
| Call Number | AIT Thesis no.FB-09-08 |
| Subject(s) | Medicinal plants--Thailand Phytochemicals--Thailand |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no. FB-09-08 |
| Abstract | The purpose of this study was to screen phytochemicals and determine the antioxidant and antimicrobial activity of two Thai herbal formulations (Triphala and Solot Benjakul) and single herbal extracts used to make these formulations by aqueous and ethanol extracion. Triphala is widely used in a large number of medicinal formulations and used to promote overall and digestive health during summer. Triphala contains equal proportion of dried fruit powder of Emblica officina lis, Terminalia chebula and Terminalia bellirica. Solot Benjakul formula is used to balance all elements in human body and composed with fruits of Piper retrofractum, roots of Piper sarmentosum, stems of Piper ribesoides, rhizomes of Zingiber officinalis and roots of Plumbago indica at ratio 8:4:3:2:1. Triphala, Solot Benjakul and their conponents were extracted with water and ethanol and shown extraction yields in the range of 8.05 to 43.92%. Comparative phytochemical profile of Triphala, Solot Benjakul and their conponents was done by preliminary phytochemical screening, thin layer chromatography fingerprint and total phenolic contents. TLC fingerprint was determined under UV 254 and 366 nm after being sprayed with ferric chloride and natural product (NP) with polyethylene glycol (PEG) 4000. It indicated all the extract possess phenolic compounds. Some extract showed the presence of flavonoids. Phytochemical screening test exhibited the presence of tannins, alkaloids, flavonoids, saponins, anthraquinones and steroids. Total phenolic contents of extracts ranged from 15.26 t0793.32 mg GAE/g extract. Biological activities of Triphala, Solot Benjakul and their conponents were done by Thin Layer Chromatography (TLC) bioassay, antioxidant activity by with DPPH (2,2' diphenyl-1-picrylhydrazyl) radical scavenging and antibacterial activity by disc diffusion and minimum inhibitory concentration assay. TLC bioassay with DPPH derivatization results confirmed the differences of phytochemical compositions with differences in polarity as well as antioxidant activity. To evaluate antioxidant activity by DPPH free radical scavenging, all extracts significantly exhibited antioxidant activity. The IC₅₀ of the extracts ranged from 3.19 to 396.11 ug/ml and IC₅₀ of standard solutions (ascorbic, ellagic and gallic acid) ranged from 1.21 to 2.84 ug/ml. Antimicrobial activities of crude extracts were tested against four strains of foodbome pathogens (Staphylococcus aureus, Escherichia coli, Bacilus subtilis and Salmonella typhimurium). It was found that inhibition zones ranged from 9.38 to 21.68 mm and minimum inhibitory concentration (MIC) values ranged between 0.39 and 100 mg/ml after incubation at 37°C for 24 h Finally, the present study revealed thet the extracts of Triphala and the combination of Triphala and Solot Benjakul were not significantly different (p>0.05) in the total phenolic contents and effectiveness of antioxidant and antimicrobial activity since this combination effect is based on physicochemical interactions among the ingredients of the component herbs |
| Year | 2009 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no. FB-09-08 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip Kumar.;Omboon Luanratana; |
| Examination Committee(s) | Athapol Noomhorm;Gallardo, Wenresti G.; |
| Scholarship Donor(s) | RTG Fellowship; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2009 |