Qualitative screening of antibiotic residues and identification of antibiotic resistant Salmonella from raw and ready to eat meat in Thailand | |
| Author | Agza, Bilatu |
| Call Number | AIT Thesis no.FB-11-17 |
| Subject(s) | Salmonella--Thailand Meat--Microbiology--Thailand Antibiotics |
| Note | A thesis submitted in partial fulfillment of the re quirements for the degree of Master of Science In Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-11-17 |
| Abstract | Raw and ready to eat chicken and beef samples (n=13 0) were collected from markets in Bangkok and nearby areas to screen antibiotic resid ues qualitatively, to assess Salmonella contamination rate and to study resistance profiles of salmonella isolates from the same samples. Drug residue determining test kit was used for screening of Tetracycline, Penicillin and Sulphonamide groups of antibiotics. Salmonella was detected and identified using the conventional culture method ISO 6579 (200 2). All the isolates were evaluated for their antibiotic resistance pattern against some co mmon antibiotics using Kirby-Bauer antibiotic discs diffusion method. Fifty one out of 130 samples (39 %) found to be antibiotic residue positive for at least one of the tested antibiotic groups. Tetracycline (28%) was the leading group of antibiotics presenti ng in more number of samples followed by sulfonamide (23%) and Penicillin (20%). The over all prevalence of salmonella in the collected samples was 9 % (12 out of 130) and this rate in chicken samples (12.9%) was higher than the beef (5%) while similar result was recorded between raw and ready to eat meat samples. Eighty three percent of the salmonell a isolates were resistant to at least two antibiotics and 67 % multidrug resistant was record ed. Most of the isolates were resistant to β -lactamase (Ampicillin, Amoxicillin) and susceptibl e to Gentamicin and Kanamycin antibiotics. Though salmonella prevalence rate obse rved in this study was lower than previous reports in the country, the high rate of a ntibiotic resistance indicated a dramatic development of multidrug resistance in meat availab le to the consumers. Thus, it is essential to apply several efforts including, obser vance of withdrawal period, awareness creation and regular monitoring of antibiotic use i n animal production. |
| Year | 2011 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-11-17 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Rakshit, Sudip Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Anil Kumar Anal; |
| Scholarship Donor(s) | Asian Institute of Technology; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2011 |