Effect of polyelectrolyte based microencapsulation on the motility and cryopreservation of Boran semen | |
| Author | Jemal, Jeilu |
| Call Number | AIT Thesis no.FB-11-18 |
| Subject(s) | Microencapsulation Polyelectrolytes |
| Note | A Thesis S ubmitted i n P artial Fulfillment of the Requirements for the Degree of Master of Science Food Engineering and Bioproce ss Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-11-18 |
| Abstract | Three experiments were carr ied out to study the ef f icacy of microencapsulation of B o ran spermatozoa for use in artificial insemination i n Ethiopia . In the first e xp eriment , the s emen w as manually encapsulated at three different concentrations (10, 2 0 and 3 0 x 10 6 sperm/ 0.25 ml) in approximately 1.5 - mm diam eter beads . The beakers containing the diluted semen were placed in the water bath of 37 o C for the whole encapsulation process. Microcapsules were incubated in vitro for 24 h at 37 0 C. Unencapsulated samples of each concentration served as controls. S mears were prepared from the microcapsules after 2, 4, 6and 24hrs of incubation to evaluate subjectively percentage of motile sperm. However, high cellular injury and damage was demonstrated in the encapsulated semen and the unencapsulated controls were alive f or longer period. In the second e xp eriment , the s emen w as manually encapsulated in a concentration of 3 0 x 10 6 sperm/ 0.25 ml) in approximately 1.5 - mm diameter microcapsul es . Overnight cooled (app. 4 0 C) 1 % (w/v) sodium alginate solution w as mixed with di luted semen in 1:4 ratios . The beaker s containing the diluted semen were placed in ice bath of < 4 ºC for the whole encapsulation process (less than 30 minutes) . M icrocapsul es were incubated in vitro for 6 h rs at 37 0 C. Unencapsulated sample s with the same c oncentration served as control. Capsule contents were evaluated for percentage of sperm motility at 10, 30 mins , 1 h , 2 hrs and 3 hrs of incubation. Motility of encapsulated spermatozoa was significantly (p>0.05) lower than Un encapsulated control at early hou r of incubation (10, 30 mins 1 h , 2 hrs) and there is no significant (p<0.05) difference at the 3 hrs of incubation between the encapsulated and the free controls. Capsule fragility was evaluated after 24 h of incubation. The manual encapsulation technique wo uld not help to control uniformity of capsules size and shape. Capsule integrity was gradually affected by sodium citrate , Viability of encapsulated spermatozoa decreases after 3hrs of incubation. In the last experiment , on cryopreservation of encapsulate d Boran semen ; efforts were made to preserve the encapsulated semen at three different temperature level - 196 0 c (filling problem in 0.25ml straws, due to higher viscosity of the encapsulated semen ) , - 20 0 c (in cryo vials) and 4 0 c in Petri dish. The semen frozen in 4 0 c in Petri dish had a better motility t han other temperature levels and it is significantly (P< 0.05) higher in motility at the third day of storage than the free control under the same condition. The r educed viability and cell injury i n the f irst exp eriment was due to damage associated with longer time of encapsulation procedure in 37 o C water bath . And it was prevented in the second experiment by encapsulating in ice bath of 4 0 c . Manual sperm m icroencapsulation procedures in ice bath of 4 0 c are compatible with sperm viability and can be adapted with little modification for use in artificial insemination. |
| Year | 2011 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-11-18 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil K.; |
| Examination Committee(s) | Rakshit, Sudip Kumar;Ahtapol Noomhorm; |
| Scholarship Donor(s) | Ethiopian Government (Rural capacity building Project); |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2011 |