Screening of bioactive extracts from plant sources for modulation of and lipoprotein metabolism | |
| Author | Shruti |
| Call Number | AIT Thesis no.FB-12-06 |
| Subject(s) | Plant bioactive compounds Mecidinal plants |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-12-06 |
| Abstract | Bioactive compounds are extra nutritional constituents of food materials which are known for their uncountable health benefits. Recently researchers have found that certain extracts from various plant sources can inhibit di gestive and metabolic enzymes. H ence they are suggested as therapeutic targets for the control of certain diseases through reduced enzyme activity. This work focuses on the modulation of such enzymes which play a very important role in the metabolism of body. Alpha amylase and Alpha glucosidase digestive enzymes have already been proven for having the potency to treat Diabetes . O ther metabolic enzymes such as Cholesteryl ester transfer protein (CETP) can also be targeted for cholester ol related diseases. This lipid tra nsfer plasma protein helps in transfer of cholesteryl esters from high density lipoprote ins (HDL) to low density lipoproteins (LDL) . Inhibition in the activity of this enzyme may cause an increase in high density lipoprot ein levels and decrease in low density lipoprotein levels and thus can enhance cellular cholesterol eff lux. The present work deals with the potential of food based bioactive extracts for modulation of digestive and plasma lipid transfer protein as an appro ach to discover novel inhibitor. These can be further targeted as therapeutic drugs in future. Certain plant raw materials were screened for alpha amylase, alpha glucosidase and CETP inhibition assays. Among which Garcinia mangostana was found to be the mo st potent inhibitor of alpha amylase and CETP enzyme. These biochemical assays were done for initial screening. Alpha amylase inhibition assay is done to screen the potent compounds for having antidiabetic properties. CETP inhibition assay is a useful assa y for discovering compounds which have potenty for prevention and management of cardiovascular diseases. Garcinia m angostana raw material was extracte d in three different solvents (methanol, dicholromethane and h exane) and screened for alpha amylase inhibi tion assay. The results obtained are 39.4 μg/ml, 11.87 μg/ml and 9.048 μg/ml respectively. The same three extracts were also screened for CETP inhibition assay but the dose response was done only for the hexane extract as others were not found to have pote nt i nhibition. Thus an IC 50 of 10.89 μg/ml is obtained and the hexane extract taken for further analysis to discover the compound responsible for the activity. TLC, HPLC, LCMS analysis were performed and comparison with reference standard concluded that al pha mangosti n is the active compound in Garcinia mangostana which is responsible for the inhibitory activity of the plant raw material . |
| Year | 2012 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-12-06 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anil Kumar Anal; |
| Examination Committee(s) | Athapol Noomhorm;Rakshit, Sudip Kumar;Senthil Duraisamy; |
| Scholarship Donor(s) | Thailand ( HM Queen); |
| Degree | Thesis (M. Eng.) - Asian Institute of Technology, 2012 |