Incorporation of isolated probiotic from fish gut in feed as functional additive for healthy and value added fish production | |
| Author | Kumaree, Kishore Krisshna |
| Call Number | AIT Thesis no.FB-12-22 |
| Subject(s) | Probiotics Microencapsulation Lactic acid Dietary supplements |
| Note | A thesis submitted in partial fulfillment of the requirements for the degreeof Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no. FB-12-22 |
| Abstract | A detail study was conducted to explore the gut microfloraof two fish species (catfish and mackerel). The isolated bacteria were divided in two groups (Probiotic and Pathogenic) according to its capabilities. The samples were collected from the local fish market. Forty colonies from the gut of both cat-fish and mackerel were randomly screened for its probiotic and pathogenic capabilities. Out of all 40 colonies only one was found of probiotic characteristics and three were found belonging to pathogenic flora. The isolated probiotic bacteria were found with highly antibacterial properties against the pathogenic bacteria. The specie was identified by API-50 kit and was confirmed as Lactobacillusplantarum. While the pathogenic bacteria flora was identification by gram staining and biochemical test. All the pathogenic bacteria were found belonging to the family Enterobacteriacea. One pathogenic bacteria with poly-unsaturated fatty acids (PUFAs) production capability was identified with the help of 16s rDNA Polymerase Chain Reaction (PCR). The identified organism was Klebsiella pneumoniea ozaenae, whilethe remaining wereKlebsiella pneumoniea pneumonia,and Vibrio parahaemolyticus.The isolated probiotic was encapsulated in calcium alginate-gelatin systems to ensure the high viability during fish feed pellets processing (high temperature) and in the fish gut (various pH and enzymes). |
| Year | 2012 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no. FB-12-22 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil K.; |
| Examination Committee(s) | Athapol Noomhorm ;Rakshit, Sudip K.; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2012 |