Enzymatic production of xylooligosaccharides from coconut husk and their prebiotic effects on growth of Lactobacillus plantarum | |
| Author | Krittayawan Bhumiwat |
| Call Number | AIT Thesis no.FB-13-03 |
| Subject(s) | Coconut products Coconut |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of S cience in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-13-03 |
| Abstract | The extraction of xylo oligosaccharides (XOS) from coconut husk fibers was performed. The prebiotic effects of XOS had done on the growth of probiotic, Lactobacillus plantarum . Alkali extraction of xylan was done using application of microwave and ultrasonic. The optimum condition of both assistant w as observed using response surface methodology (RSM). Three important parameters were NaOH concentration (2, 7 and 12% (w/v)), extraction time (5, 10 and 15 min) as well as power (100, 200 and 300 watt) or temperature (30, 45 and 60 ° C) for microwave and ul trasonic assisted extraction, respectively. The results showed that all parameters affected on % xylan recovery. The parameter that had highest effect on % xylan recovery was NaOH concentration while the extraction time has the higher effect than extractio n power or extraction temperature. The optimum rang for microwave - assisted extraction was 6.79 - 12 % (w/v) NaOH, 7 - 11.5 min of extraction time and 120 - 210 watt while the application of ultrasonic was 7.86 - 10.24 % (w/v) NaOH, 8.25 - 13.39 min of extraction tim e and 40 - 60 °C. Nevertheless, the application of ultrasonic was used to produce xylan due to obtaining higher yield , saving energy because of using lower extraction temperature as well as less loss. There was 10.23 % xylan recovery on fresh coconut husk . XOS produced from extracted xylan by enzymatic hydrolysis of endo - (1 4) - xylanase from Thermomyces lanuginosus . The optimum condition which produced highest concentration of xylo oligosaccharides solution was at temperature 60 ° C using xylanase 80 IU and h ydrolysis for 4 hours. There was 21.35 % yield of XOS powder was extracted from xylan. The XOS powder was analyzed its structure using FTIR spectrophotometer. It was found that at least in this XOS contained xylobiose. For the effect of XOS on prebiotic growth was found that the growth of prebiotice; Lactobacillus plantarum increased as well as dry cell mass with increasing XOS concentration . At 8 hour of incubation , dry cell mass of samples added 0.5, 1.0 and 2.0 mg of XOS per one ml of MRS were higher t han control 0.21, 0.28 and 0.48 g/L, respectively. I t can be indicated that XOS which extract from coconut husk fibers has a good effects on growth of Lactobacillus plantarum due to it s prebiotic properties. |
| Year | 2013 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-13-03 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Muanmai Apintanapong; |
| Scholarship Donor(s) | Royal Thai Government;Asian Institute of Technology Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2013 |