Functional properties and antimicrobial activities of banana (Musa Sapientum L) flower proteins | |
| Author | Kewalee Sitthiya |
| Call Number | AIT Thesis no.FB-13-04 |
| Subject(s) | Plant proteins Banana flower |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-13-04 |
| Abstract | The proteins and peptide were extracted from the banana flowers by alkaline using ultrasonic assisted extraction and enzymatic methods. Response surface methodology was employed to optimize the ultrasonic assisted extraction of protein from banana flowers. Optimal conditions were determined and three dimensional response surfaces were plotted using mathematical models. The analysis indicated that all the parameters, i.e. extraction temperature, extraction time and concentration o f alkali (NaOH), had significant positive linear and negative quadratic effects on the protein yield. Optimum conditions obtained were the extraction temperature 51 - 58 , extraction time 37 - 50 min and NaOH concentration 0.8 - 1.2 M while the experimental yiel d of protein was in agreement with the predicted value. It was found that an alkaline method produced a high protein yield. The use of enzyme pepsin appeared to be less e ff ective in extracting banana flower protein. Analysis of protein s purity by Fourier Tra nsform Infrared spectrometer , UV - Vis spectrometer w e r e also carried out on the extract protein from banana flower compared with bovine serum albumin (BSA), a model protein. The banana flower proteins had three subunit molecular weights of 66, 48 and 12 kDa. The extracted proteins and peptides were evaluated for antimicrobial activities by well diffusion assay. The minimum inhibition concentration of Escherichia coli and staphylococcus aureus were observed at 25 mg/ml and 5 mg/ml respectively |
| Year | 2013 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-13-04 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Muanmai Apintanapong; |
| Scholarship Donor(s) | Royal Thai Government Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2013 |