Isolation of pathogens in eggs from farms and markets in Thailand and analysis of their resistance to commercial antibacterials and lactobacilli | |
| Author | Siriporn Chaemsanit |
| Call Number | AIT Thesis no.FB-13-05 |
| Subject(s) | Eggs--Production--Thailand Lactic acid |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-13-05 |
| Abstract | Microbiological contamination of egg was strictly concern on food safety issues globally since it related to many outbreaks that posed treat to human health hazard. Due to the emerging of egg associated outbreak in Thailand, the study of microbiological contamination of egg shell and egg content at the farm layer and market layer of the egg production chain in Thailand was carried out to exposure assessment of the prevalence of flora bacterial and pathogens on egg before the risk assessment was further performed. A total 16 egg samples were collected from four different origins in farm and market layer (cage farm, cage free farm, supermarket and local market)as a case study. Total aerobic count was evaluated and compared between different sources. The three main pathogens included E.coli, E.coli O157:H7 and Salmonella were detected. Identification of contaminated bacterial was studied. Only one sample out of 16 samples was contaminated with Klebsiella oxytoca in egg content. No E.coliO157:H7 was found. Three eggs were found positive with E.coli(one from Cage free farm and two from Cage farm) and two eggs were positive with Salmonella.(one from Local market and one from Cage free farm). Totally 116 isolates from 16 egg shell samples were identified for their Gram reaction, Family and Genus. Percent isolate of bacterial one egg were determined. Moreover, with the attempt to inhibit the pathogens contamination, five antibiotic agents and three lactic acid bacterial (Lactobacilli) were examined for their antimicrobial activity toward the isolated pathogens by using disk diffusion method and agar dot assay, respectively. The flow chart of egg production chain in Thailand was established and the rick characterization between different types of farm and market was assessed before summarization of the risk assessment of microbiological contamination was concluded. |
| Year | 2013 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-13-05 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Muanmai Apintanapong; |
| Scholarship Donor(s) | Royal Thai Government Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2013 |