Effect of supportive enzymes on fermentation performance and oil recovery efficiency from corn dry-grind ethanol production | |
| Author | Pawarisa Luangthongkam |
| Call Number | AIT Thesis no.FB-14-01 |
| Subject(s) | Bioactive compounds. Enzymatic analysis Separation (Chemistry) Corn Drying |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-14-01 |
| Abstract | This study highlights the potential of fiber -hydrolytic enzymes cocktail, including protease and phytase, performance in fermentation functioning and oil recovery from dry -grind corn ethanol production. Enzymes cocktail, mainly xylanase activity, was added through production in 1.5 and 50 L fermenter at 30 °C pH 4.0 for 72 hour. Hydrolysis of cell -wall component lead to a noticeably higher production rate, solid mass, and oil partition into thin stillage was observed (P -value<0.05) with good agreement in both production scale s. Meanwhile, treatment at optimal condition of enzymes cocktail (50°C pH 5.2) had showed a better improvement at post -liquefaction incubation. The highest dry -matter yield and oil partitioning of liquid fra ction was increased by 8% and 110% compared with control. The existence of protease and phytase had provided essential nutrients that e nhance yeast activity, resulting in significantly faster production rate 1.16 g ethanol/ g dry corn h. Proteolysis activity of enzymes also enriched oil partitioning into liquid phase by hydrolyzing a outer protein layer s urrounded oil lipid as more than 77% of oil available in thin stillage. However, heat caused by d istillation stimulation showed undesirably effect on solid mass and oil partition as dropped by 2- 6% in total compared with no stimulation enzymatic treatment in the same condition . |
| Year | 2014 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-14-01 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Athapol Noomhorm |
| Examination Committee(s) | Anal, Anil Kumar;muanmai Apintanapong;Lamsal, Buddhi P. |
| Scholarship Donor(s) | Royal Thai Government Fellowship |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2014 |