Formulation of nanoemulsion of bioactive extracts in seed oil of moringa oleiferaby phase inversion and ultrasonic emulsification methods | |
| Author | Pantaree Boonpanya |
| Call Number | AIT Thesis no.FB-14-03 |
| Subject(s) | Bioactive compounds--Biotechnology Seed oil Moringa oleifera Ultrasonic |
| Note | A thesis submitted in partial fulfillment of the req uirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-14-03 |
| Abstract | Moringa oleifera leaves have been found the sources of nutrition, phytochemic als and antioxidants. In this study, nanoemulsion was used to encapsulate bioactive extracts in seed oil of Moringa oleifera through phase inversion and u ltrasonic e mulsification m ethods . The objective of this work was to study the extraction of anthraquin ones, phenolic compounds and antioxidant activity by ultrasonic - assisted extraction comparing with conventional methods and their antioxidant activity of Moringa oleifera leaves extracts. Moreover, the study of preparation of edible nanoemulsion was invest igated by low and high - energy techniques . The study exhibited that, three variable parameters ( extraction time , cycle and amplitude ) had affected on extracts. O ptimum range of bioactive extracts was : extraction time 55 to 60 min, cycle 0.97 to 1.0 and ampl itude 78 to 100 % for ultrasonic - assisted extraction method. The results showed the highest amount of bioactive plant extracts from UAE methods were higher than two conventional methods : magnetic stirring and maceration. According to the nanoemulsion form ulation, small droplets can be produced by low energy and high energy methods, but high energy method need small amount of surfactant to formulate smaller droplets. Nanoemulsions produced by emulsion phase inversion method had mean par t icle diame ter (d ˂ 1 30 nm) while ultrasonic emulsification method (d ˂ 35nm) with the narrow polydispersity indexes (PI ~ 0.095). Besides, oil and surfactant concentration had positive and negative relationship, respectively to droplets size . Degradation of phenolic compounds also studied in this experiment which showed compound degradation was slower than the control . The bioaccesibility study of phenolics and free fatty acid releasing through in vitro model conducted to stimulate the gastrointestinal fluids showed positive co rrelation to surfactant concentration s . Finally, nanoemulsion which kept for 2 weeks and 6 weeks at room temperature was applied in accelerated palm oil, the results shown decreasing of acid value and free fatty acid from heat - oil compare d to control. |
| Year | 2014 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-14-03 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Athapol Noomhorm; |
| Examination Committee(s) | Anal, Anil Kumar;Muanmai Apintanapong; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2014 |