Optimization of the extraction of bioactive bompounds from eettle leaves and their bioencapsulation for controlled gastrointestinal release | |
| Author | Mridula Thapa |
| Call Number | AIT Thesis no.FB-14-04 |
| Subject(s) | Bioactive compounds Leaves Nettle leaves Bioencapsulation |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-14-04 |
| Abstract | In this study, ultrasound - assisted extraction and microwave - assisted extraction were used for leaching the bioactive compounds from dried nettle leaves. The maximum phenolic content obtained from ultrasonic - assisted extraction was 33.43 mg GAE/g DW . Microwave - assisted extraction was found to be more effective than other conventional methods for the extraction of polyphenols from nettle leaves. 61.9mg GAE/g DW of polyphenol was extracted in 10min of microwave extraction using power supply of 100W. For the protein yield from alkaline ultrasound extraction, the maximum yield was 35.81 mg/g DW at 60°C, 30 min and 1.5 M of NaOH. All the extraction methods were optimized. The proximate, qualitative and quantitative phytochemical analysis of the dried nettle leaves were carried out. The presence of phytochemicals helped to understand the nutritional and pharmacological significance of the nettle plant. The nettle extracts were encapsulated using the calcium - alginate - chitosan system to investigate the influence on encapsulated bioactive extracts and their controlled release in simulated gastrointestinal system. The stability and encapsulation efficiency was found to be higher of about 75% in the multilayered beads, externally coated by chitosan. However, in the simulated gastric fluid (SGF, pH 1.2), the lower release of polyphenols was observed in both the single - layered calcium - alginate system and the double - coated with chitosan beads |
| Year | 2014 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. FB-14-04 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Muanmai Apintanapong; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2014 |