Extraction and characterization of collagen to develop biopolymeric fiber from chicken feet. | |
| Author | Dhakal, Damodar |
| Call Number | AIT Thesis no.FB-15-09 |
| Subject(s) | Bioactive compounds. Biopolymers. Functional foods. |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. FB-15-09 |
| Abstract | Collagen was extracted sequencially from the chicken feet using papain, acetic acid and NaCl solution. The optimum condition was found to be 30 ⁰ C temperature treatment and ( 25 ml/mg ) 0.9N NaCl concentration . The incubation time has not significant effect on yield of collagen . The highest yield ( 32.16% ) was found by the enzymatic hydrolysis for 28hr at 30 ⁰ C and (25ml /mg) of 0.9 N NaCl concentration. At lower temperature (4 ⁰ C) of incubation, the yields were less. The molecular weight of 150kDa and 250kDa and absorbance at 220 -230nm of collagen indicates 99% purity . The FTIR shows the presence of native structure of collagen. The linear decreasing trend in the relative viscosity of chicken feet collagen was seen with the increased temperature. Scanning Electron Microscopy ( SEM ) reveals the interconnected network of porous structure of collagen. The slight shifting of all the peaks of FTIR is the concrete evidence of interactions that occurs between the Polycapralactone (PCL) and the collagen. The Minimum Inhibitory Concentration ( MIC ) for Ag -PCL -collagen nanofibers was found to be 0.5 mm at 10 mg/ml for Ecsherichia coli and 0.5 mm at 20 mg/ml for Salmonella typhimurium . The combination of silver -collagen -Polycapralactone fibers could be used as a bio packaging materials. |
| Year | 2015 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-15-09 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Athapol Noomhorn;Nguyen, Loc Thai;Pisut Koomsap; |
| Scholarship Donor(s) | Her Majesty Queen’s Schol arship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2015 |