Isolation and identification of caffeine degrading bacteria from Chiangmai's coffee plantation area in Thailand. | |
| Author | Win Yi Yi |
| Call Number | AIT Thesis no.FB-15-10 |
| Subject(s) | Bioactive compounds. Microbial biotechnology. Coffee processing |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no.FB-15-10 |
| Abstract | Popular consumption of caffeine containing beverages in daily life not only adversely affects human health, but also may contaminate surrounding water. Decaffeination has been on demand these days in order to cut down caffeine contamination and to produce caffeine free products, especially coffee and tea beverages since ―coffee drinking behavior and habit‖ survey showed that 87% of residents of AIT campus like to drink coffee in their daily life. However, since conventional decaffeinating methods are expensive and sometimes involving toxic chemical agents, microbial caffeine degradation method has been emphasized and developed. Hence, caffeine degrading microbes take part in important role for bio-decaffeination. In this study, caffeine tolerant bacteria were isolated from the soil of coffee growing area. According to 16SrDNA analysis, it was identified as Burkholderia anthinawith the highest similarity 99.93% at nucleotide sequences among Burkholderia speices. It can tolerate high level of caffeine up to 20g/land can degrade 44% and 38% of caffeine content in media containing 5 g/land 10g/lof caffeine, and 14% and 10% at 15g/l and 20 g/lof caffeine containing media during 120 hours. Thus, Burkholderia anthina can be effectively used to degrade caffeine from agro-industrial wastes. |
| Year | 2015 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-15-10 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Athapol Noomhorm;Loc Thai Nguyen; |
| Scholarship Donor(s) | Deutscher Akademischer Austausch Dienst (DAAD), Germany;Asian Institute of Technology Fellowship; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2015 |