Prevalence and identification of antibiotic resistant genes in escherichia coli and salmonella isolates from green leaf lettuce in Thailand and Cambodia. | |
| Author | Chhay Chanseyha |
| Call Number | AIT Thesis no.FB-16-03 |
| Subject(s) | Salmonella--Thailand Salmonella--Cambodia Antibiotics |
| Note | A thesis submitted in partial fulfillment of the requirements for the deg ree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-16-03 |
| Abstract | The objective of this study was to evaluate the prevalence and identification of resistant genes in Escherichia coli (E. coli) and Salmonella isolated from green - leaf - lettuce (n=120), collected from open markets and su permarkets of Cambodia and Thailand. From 120 samples of lettuce leaves, 47 (39.17%) E. coli and 28 (23.33%) Salmonella were isolated and identified by biochemical and immunological tests. Among 47 isolates of E. coli , 27 (57.45%) were found multi - drug ant ibiotic resistant (MAR), whereas, among 28 Salmonella isolates, 13 (46.43%) were resistant to at least one of the tested antibiotics. E. coli were frequently resistant to amoxicillin and ampicillin (25 isolates, 92.6%), followed by tetracycline (19 isolate s equivalent to 70.4%). Similarly, Salmonella were frequently resistant to ampicillin and amoxicillin, (13 isolates, 100%), followed by tetracycline (9 isolates, 69.23%). Furthermore, antibiotic resistant E. coli and Salmonella exhibited resistant to 800 p pm of chlorine. All antibiotic resistant E. coli and Salmonella isolates were subjected to identification of antibiotic resistant genes. The beta - lactam ( bla TEM ) and tetracycline ( tetA and tetB ) resistance genes were identified by polymerase chain reaction in the tested bacterial isolates. This study confirmed the presence of antibiotic resistant strains of E. coli and Salmonella in lettuce leaves that could be a potential threat to consumer’s health as lettuce leaves are commonly used as salad. The high pr evalence and resistance of E. coli and Salmonella isolated from lettuce leaves highlighted the need to improve and adopt hygiene practices in order to ensure the safety of consumers. |
| Year | 2016 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-16-03 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anil Kumar Anal; |
| Examination Committee(s) | Athapol Noomhorm;Loc T hai Nguyen; |
| Scholarship Donor(s) | Deutscher Akademicher Austausch Dienst (DAAD); |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2016 |