Development of an electrochemical sensor for detection of tetracycline using molecularly imprinted overoxidized polypyrrole and gold nanoparticles | |
| Author | Devkota, Lavaraj |
| Call Number | AIT Thesis no.FB-16-08 |
| Subject(s) | Electrochemical sensors Tetracycline Molecular imprinting |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no.FB-16-08 |
| Abstract | A selective and sensitive electrochem ical sensor for tetracycline detection was fabricated using low cost screen printed carbon electrode (SPCE) modified with molecularly imprinted overoxidized polypyrrole (MIOPPy) and gold nanoparticles (AuNP) . Molecularly imprinted polypyrrole ( MIPPy ) was p repared by electropolymerization of pyrrole in the p resence of tetracycline as template. The template removal was achieved by applying a fixed potential of 1.0 V for 30 minutes in 0.1 M H 2 SO 4 and overoxidation was done in the presence of 0.05 M NaOH. The e lectrochemical and morphological characterization of modified sensor was done using differential pulse voltammetry and scanning electron microscopy respectively. The electrochemical behavior of tetracycline on MIOPP y - AuNP/SPCE was studied using DPV in the presence of sodium dodecyl sulfate. The most important parameters governing sensor performance were studied and optimized. Tetracycline (TC) oxidation peak was shown to be enhanced considerably due to the synergistic effect of AuNP and MIP. Furthermore exc ellent electro catalytic activity of TC was exhibited by the use of anionic surfactant, sodium dodecyl sulfate . The sensor showed good selectivity against three common antibiotics used in agriculture , oxytetracycline, chloramphenicol and amoxicillin . Under optimized conditions the sensor showed a line a r range at 1 to 20 μ M of TC with limit of detection of 0.65 μ M. The peak current responses were highly reproducible with relative standard deviation of 3.43 % and under 15 days storage at room temperature sign al deviation was as low as 0.78 % indicating high stability. It was observed that food matrix had certain interference on the sensor performance. Therefore an appropriate sample preparation technique was recommended. The sensor was successfully used to det ect spiked tetracycline in fruit juice and water from fish pond with good recovery. |
| Year | 2016 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-16-08 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Loc Thai Nguyen; |
| Examination Committee(s) | Athapol Noomhorm;Anal, Anil Kumar; |
| Scholarship Donor(s) | Thailand ( H.M Queen ); |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2016 |