Bioactive extract from xanthium strumarium L. fruit and in vitro evaluation of biological activiities and their relation with redox properties. | |
| Author | Ingawale, Amol Subhash |
| Call Number | AIT Thesis no.FB-16-09 |
| Subject(s) | Bioactive compounds. Plant bioactive compounds Mecidinal plants Botanical chemistry Phytochemicals |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. no.FB-16-09 |
| Abstract | In this study, Xanthium strumariumL. fruit was extracted and the product obtainedwas analyzed for its antioxidant, antimicrobial and anti-diabetic activity. In addition, electroactivity of the extracted compounds was determined by electrochemical methods and its correlation to the bioactivity was explored. The effects of solvent (methanol), extraction time and solid to solvent ratioon total phenolic content (TPC), 1,1-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity and the ferric reducing antioxidant power (FRAP) were investigated. The results indicated that optimal methanol concentration, extraction time and solid to solvent ratio were 60%, 10 min and 1:12.5, respectively. Corresponding TPC, DPPH and FRAP were 9.25 GAE/g of sample, 28.5 dpph/0.1g of sample, 54.7 μM of Fe (II)/g of sample, respectively. Methanol concentration and solid to solvent ratio were found to have significant effects on the TPC, DPPH and FRAP of the extract. Extracted compounds demonstrated antibacterial activity against selected bacteria. When tested against α-glucosidase, Xanthium strumariumL. fruit extract exhibited strong inhibitory activity and the effects increased with increasing extract concentration. Electrochemical measurement showed that overall oxidation peak of the compounds present in the extract had good correlation with the concentration but not with anti-diabetic activity. The study, to a certain extent, revealed important bioactivity and redox behavior of Xanthium strumariumL. fruit extract which could be used in future applications. |
| Year | 2016 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-16-09 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Loc T. Nguyen; |
| Examination Committee(s) | Athapol Noomhorm;Anal, Anil Kumar; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2016 |