Sequential exraction of hyaluronic acid and collagen from chicken eggshell membrane and formation of chitosan-hyaluronic acid-collagen based biopolymeric nanoparticles | |
| Author | Alparce, Nina Karla Mallorca |
| Call Number | AIT Thesis no.FB-14-09 |
| Subject(s) | Extraction Hyaluronic acid Collagen Biopolymers--Biotechnology |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resource and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis;no.FB-14-09 |
| Abstract | Hyaluronic acid (HA) and collagen were sequentially extracted from eggshell membrane. HA was extracted with salt solutions and by papain digestion. In the former, the yield initially increased with salt concentration, the highest being 0.88 mg/g sample using 0.5 M NaCl solution, but decreased thereafter. This method was optimized by B ox and B ehnken experiment s varying the salt concentration s , extraction period , and volume of the extraction solvent . It was predicted that extraction with 1.5 M NaCl solution at a sample to sol vent ratio of 1:40 for 53 min will yield as much as 0.98 mg /g sample . In papain digestion, the highest yield, 5.48 mg/g , was obtained from samples incubated at 60 ⁰ C. The yield s were less at lower incubation temperatures. Collagen was extracted from the samples with acetic acid solution s of varied pH . The maximum yield was obtained at pH 2. But sample pretreatment by ultrasonication resulted in higher yields. The most significant improveme nt was observed in samples sonicated at 75% amplitude. Meanwhile, the highest collagen yields were obtained from residual samples recovered after papain - digestion. Amount of collagen extracted from samples digested at 20 ⁰ C was about 38 mg/g sample but decreased with increasing incubation temperature due to collagen denaturation . Collagen and HA are both capabl e of forming complexes with other polymers through electrostatic interaction. HA solution combined with chitosan solution instantaneously form ed nanoparticles. However, mixing chitosan with collagen solution and collagen with HA solution resulted in large scale aggregation and formation of very large particles with hydrodynamic diameters in the micrometer range. |
| Year | 2014 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis;no. FB-14-09 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Athapol Noomhorn; |
| Examination Committee(s) | Anil Kumar Anal;Muanmai Apintanapong; |
| Scholarship Donor(s) | Asian Development Bank - Japan Scholarship Program; |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2014 |