Optimization of ultrasound assisted extraction (UAE) and evaluation of physiochemical and bioactive properties of extracts and resistant starch from black glutinous rice and its bran | |
| Author | Pariyarath, Anjali Maniyam |
| Call Number | AIT Thesis no.FB-18-08 |
| Subject(s) | Extraction (Chemistry) Bioactive compounds--Biotechnology Ultrasonic waves |
| Note | A thesis submitted in partial fulfillment of the requirements for the Degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no.FB-18-08 |
| Abstract | Black glutinous rice, as well as its rice bran, are very rich in anthocyanins and other bioactive compounds. Anthocyanins provide the pigmentation to the rice and rice bran. Ultrasonic Assisted Extraction (UAE) was mainly utilized for extracting of bioactive compounds, in black glutinous rice. Effects of three independent variables; ethanol concentrations, amplitudes and times in the response factor such as extraction yield, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity and reducing power the bioactive compounds the black glutinous rice and also its rice bran were optimized by using response surface methodology (RSM). The best conditions for extraction from, black glutinous rice as predicted by RSM were 70% ethanol (v/v), 65% of amplitude and 5 min of extraction time. The anthocyanins content at these conditions was 58 mg of cyanindin - 3 – glucosidase /100g. In addition to, the best ultrasonic - assisted conditions for phenolic, antioxidant and reducing power were entrenched as extraction time of 5 min, 65 % of amplitude, and ethanol concentration of 70%. Total phenolic content (TPC) extract array from 17.42 – 179.42 mg GA E/ g and total flavonoid content (TFC) of 7.51 - 99.97 mg quercetin equivalent/g respectively. Furthermore, reducing power of extract was obtained 7.24 – 189.58 mg AAE/g and DPPH activity array in between 38.89 – 55.9% respectively. Additionally, the anthoc yanin content was in the range of 10.02 – 53.77 mg cyaniding - 3 - glucoside/100 g, total phenolic content (TPC) exhibited by extracts were 65.85 – 136.17 mg GAE/ g and total flavonoid content (TFC) ranged from and 0.13 – 2.59 mg quercetin equivalent/ g res pectively. Thus, the antioxidant activity DPPH was 57.84 – 80.14, and reducing power, of extract was between 38.9 – 239.94 mg AAE/ g respectively. For the extraction of resistant starch from the black glutinous rice from the extraction condition 100:400, 1 00:800 and 100:1200. The resistant starch content was shown higher in the 1:8, and the RS content was 12 g/100g. The maximum inhibition percentage shown by resistant’s starch as 28%, compared with control resistant’s starch shown glucose concentration very less. In case of prebiotic effects, Lactobacillus showed higher growth at the 2 % of resistant starch in media |
| Year | 2018 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-18-08 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Food Engineering and Bioprocess Technology (FB) |
| Chairperson(s) | Anal, Anil Kumar; |
| Examination Committee(s) | Loc Thai Nguyen;Datta, Avishek;Sadiq, Muhammad Bilil; |
| Scholarship Donor(s) | Asian Institute of Technology Fellowship; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2018 |