1 AIT Asian Institute of Technology

Effects of light intensity and photoperiod time for biomass production of chlorella vulgaris

AuthorLal, Aditya
NoteA thesis submitted in partial fulfilment of the requirements for the degree of Master of Engineering in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development
PublisherAsian Institute of Technology
AbstractThe study was aimed to cultivate Chlorella vulgaris for enhanced biomass production in lab-scale illuminated rack system at different light intensity and photoperiod time. BG11 media was used for rapid growth. Initial study was on turbidity, pH and average biomass dry weight for biomass production under HLI (High light intensity) with four light lamps provided 6050 Lux, MLI (Medium light intensity) with three lamps provided 4320 Lux and LLI (Low light intensity) with two lamps provided 2850 Lux . Results revealed MLI was the optimum condition followed by LLI and HLI respectively. Further in discrete study of 16, 18 and 20 hours of photoperiod time, 16 hours was found optimal for MLI, LLI and HLI respectively for biomass production of Chlorella vulgaris. Collectively MLI with 16 hours of photoperiod time was found to be the optimized condition. Under this cultivation condition the exponential cycle was of 20 days. The remained optimum condition was based on the determination of protein, total carbohydrates, total lipid and chlorophyll. LLI was second best condition of cultivation for overall study in the rack system. Whereas for antioxidant activity relating to total phenolic content the LLI had depicted optimal results in % reduction of DDPH and similar was the condition for TPC followed by MLI and HLI respectively. Photoperiod time of 16 hours for cultivation condition had important significance for TPC in metabolism for antioxidant activity. .The end product biomass dry powder was 1.58 g at 16 hours of photoperiod time, 1.35 g at 18 hours photoperiod and 1.27 g at 20 hours in MLI. For HLI 1.24 g at 16 hours, 1.17g for 18 hours and 0.998 g for 20 hours. And for LLI 1.21 g at 16 hours, 1.16 g at 18 hours and 1.03g at 20 hours respectively
Year2013
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB))
Academic Program/FoSFood Engineering and Bioprocess Technology (FB)
Chairperson(s)Anal, Anil Kumar ;
Examination Committee(s)Athapol Noomhorn;Muanmai Apintanapong;
Scholarship Donor(s)Sam Higginbottom Institute of Agriculture, Technology & Sciences, India. ; AIT Partial Fellowship;


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