Evaluation of selected pre-processing methods to improve digestibility of enzyme treated feather meal and enhance growth performance of Nile Tilapia (Oreochromis niloticus) | |
| Author | Herath, Sandamali Sakunthala |
| Call Number | AIT Thesis no. AQ-10-13 |
| Subject(s) | Nile tilapia--Feeding and feeds Nile tilapia--Growth Fishes--Feeding and feeds |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Aquaculture and Aquatic Resources Management, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-10-13 |
| Abstract | Two experiments were conducted to evaluate the effects of particle size reduction and fermentation (with Saccharomyces cerevisiae) of enzyme treated feather meal on growth performance, feed utilization efficiencies and digestibility of Nile tilapia juveniles. Enzyme treated feather meal was separated into three groups according to particle size as un-sieved (EFM), particles < 850μ (EFM-850), particles < 425μ (EFM-425). Each group was fermented for 24h to get fermented un-sieved (FEFM), fermented 850μ (FEFM-850) and fermented 425μ (FEFM-425) ingredients. For growth trial, a control diet was prepared with 10% fish meal as the sole source of animal protein. Six experimental diets were prepared by fully replacing fish meal component in control diet using same proportion of one of six types of key ingredient. Sex reversed male Nile tilapia (6g) was used for the experiment and lasted for 56 days. In vivo digestibility of ingredient was tested using fish meal based reference diet and six test diets which were prepared by using 70% of reference diet and 30% of tested ingredient with 0.5% of chromic oxide. In vitro digestibility was measured using crude enzymes extracted from whole gut of Nile tilapia. Results of growth trial revealed that EFM-425 and FEFM treatments had the significantly highest live weight gain, and DWG while the poorest growth performances was observed in fish fed FEFM-425. The feed intake was significantly influenced by the treatment and highest and lowest intake reported for FEFM and FEFM-425 respectively. The FCR ranged from 1.2 to 1.4 where lowest value reported for EFM-425 and it was significantly lower compared to control. PER ranged from 2.25 to 2.55 and it was not affected by treatment. ANPU of this study was slightly affected by treatment where EFM-425 and FEFM were significantly superior to that of others. Survival was above 95% in all the treatments and was not statistically significant. Analysis of final body carcass showed that only the ash content was affected by treatment. Results of both in vivo and in vitro digestibility experiments were almost similar and un-sieved enzyme treated feather meal (EFM) had the significantly poorest protein digestibility while dry matter digestibility was significantly lower in both EFM and FEFM- 425. Both digestibility experiments showed that EFM-425 and FEFM had significantly highest protein and dry matter digestibility among tested ingredients. Linear regression showed that there was a strong positive correlation (r2=0.97) between in vivo and in vitro digestibility as well as dry matter digestibility and daily weight gain (r2=0.71). This study showed that either particle size reduction up to 425μ or fermentation (without sieving) can be used as methods to improve digestibility of enzyme treated feather meal and enhance growth performance and feed utilization efficiency of Nile tilapia juveniles. |
| Year | 2010 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-10-13 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Agricultural and Aquatic Systems(AS) |
| Chairperson(s) | Yakupitiyage, Amararatne ; |
| Examination Committee(s) | Gallardo, Wenresti G. ;Thakur, Dhirendra P. ; |
| Scholarship Donor(s) | Ministry of Foreign Affairs, Norway; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2010 |