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Enzymatic conversion of coconut pulp residue into microfibrillated cellulose and its stabilizing effect on food emulsion | |
Author | Budiyantoro |
Call Number | AIT Thesis no.FB-19-03 |
Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Engineering and Bioprocess Technology, School of Environment, Resources and Development |
Publisher | Asian Institute of Technology |
Series Statement | Thesis ; no.FB-19-03 |
Abstract | The cellulose from coconut residue was extracted by an environmental friendly process. A series experiment were conducted to optimize the alkaline treatment KOH and the cellulose isolation through enzymatic hydrolysis using xylanase enzyme. A fibrillation process using ultrasonic treatment in 200 W 24 kHz for 20 min was applied for fragmentation the cellulose into microfibrillated cellulose (MFC). The response surface method (RSM) with Box Behnken design was used to optimize the variables for both, alkaline treatment and enzymatic hydrolysis. The characteristics of MFC in o/w emulsion were analyzed via evaluating the creaming index, viscosity and the stability of emulsion. The MFC of coconut residue was obtained from this have diameter and length ranging 12.7 nm to 31.3 nm and 300 nm to 500 nm and poly dispersity index being more than 0.5. Furthermore the MFC of coconut residue have been extracted from coconut residue as much as 12.5% yield with 10% (w.b) solid content. Duration of enzymatic hydrolysis was found to have effect on the cellulose content of MFC. Optimum condition was obtained with 20U/g of enzyme concentration and 36 h duration for hydrolysis which produced the cellulose content of 78.78%. The MFC of coconut residue also able to maintain the stability of o/w emulsion which has water oil ratio 90:10, in 0.2% concentration MFC in the continuous phase. All concentration MFC range 0.025-0.2%(w/v) able to form and stabilize the o/w emulsion without any serum and oil separation during storage for 7 d. The stability of MFC in o/w emulsion was indicated by high magnitude of zeta potential, -51.53 mV. |
Keyword | Cellulose; emulsion; microfibrillated cellulose; KOH; Response surface methodology; xylanase, ultrasonic |
Year | 2019 |
Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. no.FB-19-03 |
Type | Thesis |
School | School of Environment, Resources, and Development (SERD) |
Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
Academic Program/FoS | Bioprocess Technology (BP) |
Chairperson(s) | Loc Thai Nguyen; |
Examination Committee(s) | Anal, Anil Kumar;Datta, Avishek ; |
Scholarship Donor(s) | Indonesian Agency for Agricultural Research and Development (IAARD) ; |
Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2019 |