| Author | Julianingsih |
| Call Number | AIT Thesis no.PH-00-13 |
| Subject(s) | Soy oil
|
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of
Engineering |
| Publisher | Asian Institute of Technology |
| Abstract | A study was conducted to evaluate the effect of conventional pretreatment (size reduction and thermal treatment) and the use of enzymatic hydrolysis to enhance the oil extraction by using mechanical and solvent extraction. Thai soybean of SJ-5, which is widely used in vegetable oil industry, was used. Carbohydrase enzymes, Celluclast 1.5 L and Viscozyme 120 L, were used to hydrolyze the bean. The operating concentration and condition of both enzymatic treatments on oil yield were investigated. A scaled up laboratory extractor was developed to extract sufficient oil and evaluate the oil quality aspects including free fatty acid and peroxide value. The total extractable oil ofSJ-5 soybean was 25.17 ± 0.23 % based on 24 hours Soxhlet solvent extraction. Conventional pretreatment gave an effect on oil yield for mechanical and solvent extraction procedures. The smaller particle sizes gave higher oil yield. Steaming treatment gave positive effect on oil yield but drying gave negative effect on the oil yield. Conventional pretreatment in conjunction with enzymatic treatment helped to break the soybean cell tissue and complex molecules releasing the oil and enhanced the oil yield up to 1.05 % and 3.29 % by using mechanical and solvent extraction. Enzymatic incubation by using 1 % Celluclast 1.5 L with 24 % moisture content for 24 hours incubation gave the highest extractable oil by using mechanical extraction in this experiment. The recovery of extractable oil was 33.37 %. Enzymatic incubation followed by Soxtec solvent extraction gave highest recovery of extractable oil. Enzymatic treatment by using Celluclast 1.5 L, Viscozyme 120 L gave 96.15 % and 95.07 % of the recovery of extractable oil, respectively. Optimum condition for enzymatic treatment by using Soxtec solvent extraction was 50°C, 24 % moisture content during incubation for 24 hours incubation and 1 % v/w of Celluclast 1.5 L enzyme concentration or 12 hours incubation and 2 % v/w of Viszoyme 120 L enzyme concentration. Conventional pretreatment and its conjunction with enzymatic treatment gave no adverse effect on oil quality such as free fatty acid and peroxide values. All of the free fatty acid and peroxide values were lower than the standard value for soybean oil quality. |
| Year | 2000 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Postharvest and Food Process Engineering (PH) |
| Chairperson(s) | Athapol Noomhorm ;Rakshit, Sudip; |
| Examination Committee(s) | Jindal, V. K.; |
| Scholarship Donor(s) | Queen's Scholarship; |
| Degree | Thesis (M. Eng.) - Asian Institute of Technology, 2000 |