Isolation and examination of copper-removing bacteria from activated sludge culture for the development of an advanced metal removal process | |
| Author | He, Xuan |
| Call Number | AIT Thesis no.EV-00-5 |
| Subject(s) | Sewage--Purification--Activated sludge process Sewage--Purification--Heavy metals removal |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering |
| Publisher | Asian Institute of Technology |
| Abstract | The accumulation of heavy metals in water and soil is of growing concern because of health risks posed by human and animal exposure. Recent research in the area of heavy metal removal from wastewaters and sediments tries to reduce metal concentration to the permissible levels with appropriate methods. Development of bacteria-based biosorbents to increase affinity, capacity and selectivity for target metals is a promising technology. There is a demand for using genetically engineered bacteria to produce heavy metal complexing proteins, which play an important role in industrial wastewater treatment plant. This research was focused to isolate metal removing bacteria from activated sludge culture and test the metal removal capability of isolated bacteria. Experimental data showed that isolates could be chosen by using non-selective medium (NSM) in the presence of copper. There were two concentrations of copper solution (CuCb) used to discriminate the copper-tolerance level of each isolate-0.SmM and 2.SmM. The isolates were displayed on agar plates with diverse colonial morphology characteristics. Copper uptake study was conducted and reported. A maximum uptake of 8lmg/g dry cell mass was observed at an initial copper concentration of 50mg/I. The minimum uptake was 9mg/g dry cell mass. Copper uptake efficiency by isolates from NSM with O.SmM CuCl2 was significantly different with those from NSM with 2.SmM CuCb. This observation indic~ted that biological response for accumulation of copper was associated with the initial defined media, which provided a growth parameter for coppertolerant bacteria. Some isolates appear to have the potential to be an effective absorbent for the removal of copper from polluted water. It is suggested in the future work to develop the "seed" of these promising bacteria and test their conditions to be utilized in wastewater treatment plant. |
| Year | 2000 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC)) |
| Academic Program/FoS | Environmental Engineering and Management (EV) |
| Chairperson(s) | Fukushi, Kensuke |
| Examination Committee(s) | Samorn Muttamara;Preeda Parkpian |
| Scholarship Donor(s) | Her Majesty Queen's Scholarship, Thailand |
| Degree | Thesis (M.Eng.) - Asian Institute of Technology, 2000 |