Isolation of fungal chitin deacetylase enzyme producing fungus | |
| Author | Tin Tin Hlaing |
| Call Number | AIT Thesis no. BP-00-04 |
| Subject(s) | Fungal enzymes Biotechnological microorganisms--Isolation Chitin |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. BP-00-04 |
| Abstract | In this study we have investigated the chitin deacetylase activity of a number of new fungal isolates from the rain forest. These strains have been identified as insect pathogenic fungi, which are able to penetrate through the fungal cuticle and therefore might be especially equipped to break down the crystalline chitin in the cuticle. So far, a group of twenty fungi have been investigated. They appear to grow slowly. In the cell free extracts of three of these strains, evidence has been found for enzymes capable to deacetylate chitin or partially deacetylated chitin. Some enzymes are more effective in the deacetylation of chitin as compare with partially deacetylated chitin as substrate. The slow growth of those insect pathogen fungi has shown to be a large handicap for the study of the presence of CDA. Therefore additional nitrogen sources have been tested for their ability to stimulate growth. For eight out of twenty-one (8/21) strains no effect of addition of peptone or yeast extract was found; the other strains showed better growth. In the two strains that showed the highest growth rate, no CDA was found. Six out of twenty one (6/21) strains show small amount of release of CDA, their activity increase when inducer is used. Among those strains Paecilomyces amoeneroseus and Paecilomyces tenuipes have the ability to degrade natural chitin. These CDA's cloned into industrial production strains may be useful for the development of enzymatic deacetylation of chitin at industrial scale. |
| Year | 2000 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BP-00-04 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Stevens, Willem F.; |
| Examination Committee(s) | Suwalee Chandrkrachang;Rakshit, Sudip K.;Pakom Nuchnoi; |
| Scholarship Donor(s) | Asian Institute Of Technology; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2000 |