| Author | Selvarajah, Vilashini |
| Call Number | AIT Thesis no. BP-02-14 |
| Subject(s) | Petroleum--Refining
|
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Masters of
Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. BP-02-14 |
| Abstract | An enzyme Phospholipase A I (lecitase Novo) from microbial source was used to reduce
the phospholipid (PL) level below I 0 ppm to enable the physical refining for discerning
consumers. This would overcome the problem of using chemical catalysts or porcine
enzymes for this process.
At first water degumming was optimized with amount of water, temperature, time,
agitation and shaking as parameter. The results revealed that 40 C, 3% water and agitation
for 30minutes seem to be the best condition among the all combination of water
degumming process in soybean oil. For rice bran oil 2% water was sufficient to reduce the
gum to satisfactory level at 55 C with agitation for 15 minutes.
Then enzyme and water was used together using agitation and shaking for mixing at 40 C.
The results revealed that in case of soybean oil, shaking (reciprocatory at 200 times per
minute) took 12 and 14 hours with the enzyme dosage of 1200 and 720 LENU/I
respectively to obtain degumming of oil below 10 ppm phosphatides. No positive results
(I 0 ppm) were obtained using agitation within 15 hours of incubation period and up to the
enzyme dosage of 1200 LENU/1. Then water and enzymatic degumming was done in 2
step process where further reduction of time to 6 hours with the enzyme dosage of 1200
LENU/l using shaking was obtained.
For rice bran oil 3 hours is enough to reduce the PL level to below 10 ppm with the dosage
of 720 LENU/I in c_ombined process (single step).
The experiments indicate that the enzyme was able to degum soybean and rice bran oil to
the required level. However, mixi ng is an important parameter, which will affect larger
scale processes. |
| Year | 2002 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BP-02-14 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Rakshit, Sudip K.; |
| Examination Committee(s) | Jindal, Vinod K. ;Athapol Noomhorm ; |
| Scholarship Donor(s) | The Royal Netherlands Government ; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2002 |