1 AIT Asian Institute of Technology

Microbial community structure by respiratory quinone profile at intermittently aerated membrane bioreactor

AuthorPiyaporn Songprasert
Call NumberAIT Thesis no.EV-02-22
Subject(s)Membrane reactors
Microbial biotechnology
NoteA thesis submitted in partial fulfillment of the requirements for the degree of Master of Engineering. School of Envirorunent, Resources and Development
PublisherAsian Institute of Technology
Series StatementThesis ; no. EV-02-22
AbstractIn this study, microbial community structure in intermittently aerated membrane bioreactor (intermittently aerated MBR) was observed by the respiratory quinone profiles in domestic wastewater. Differences in each quinone profile were identified by various numerical analysis such as microbial diversity, equitability and dissimilarity. Intermittently aerated MBR was composed of anoxic and aerobic conditions in the single reactor with 90 min (step 1) and 60 min. (step 2) of aeration and suction time, for 150 min in each cycle. COD removal efficiency was 93% at both steps while TN removal were 49 and 61% for step 1 and step 2 respectively. TP efficiency were found to be 27% and 30% for step 1 and step 2 experiments respectively. Dominant quinone were UQ-8 followed by UQ-10 and MK-6 at step 1 while UQ-8, MK-6 and MKĀ·lO(H4) for step 2 at both anoxic and aerobic conditions. Recommended quinone structures are found to be recommended that Pseudomonas sp., Bacillus sp., Nocardia sp., Nitrsomonas europaea, Nitrobacter winogradskyi, Streptomysces Micrococcus sp., Thiobacillus sp. and Achrobacter. Microbial diversity increased with the time of anoxic condition while microbial equitability did not show any significant difference. Dominant quinone types of attached microorganisms were UQ-8 UQ-10 and MK-8(H4) while those of suspended microorganisms were UQ-8 followed by UQ-10 and MK-8. Different quinone types between attached and suspended microorganisms were UQ-9, MK-7, MK-8(H2), MK-8(H4) and MK-IO(H4). Pseudomonas vesicularis, Staphylococcus warneri, Arthrobacter sp., Rhodococcus sp. and Nocardia sp. are suggested to the cause of the biofouling microorganisms. The microbial diversity of attached and suspended microorganisms calculated based on the composition of all quinone species were 10.90 and 9 .51, respectively and equitability of suspended microorgannism was less than attached microorganism. Dissimilarity was 0.26
Year2002
Corresponding Series Added EntryAsian Institute of Technology. Thesis ; no. EV-02-22
TypeThesis
SchoolSchool of Environment, Resources, and Development (SERD)
DepartmentDepartment of Energy and Climate Change (Former title: Department of Energy, Environment, and Climate Change (DEECC))
Academic Program/FoSEnvironmental Engineering and Management (EV)
Chairperson(s)Lee, Seung-Hwan
Examination Committee(s)Ahn, Kyu-Hong ;Chongrak Polprasert ;Trankler, Josef
Scholarship Donor(s)Goverment of Japan
DegreeThesis (M.Eng.) - Asian Institute of Technology, 2002


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