| Author | Wee, Martin A. |
| Call Number | AIT Thesis no. BP-01-09 |
| Subject(s) | Microalgae--Cultures and culture media
|
| Note | A thesis submitted in partial fu lfillment of the requirements for the degree of Masters of
Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. BP-01-09 |
| Abstract | This study had two major phases of work to determine the extent of effect of light and
temperature for better growth performance and the development of some important
polyunsaturated fatty acids (PUF A) in the cells of the freshwater microalgae. Growth studies
(the first phase of the experiment) of four species of freshwater algae, Chlorella vulgaris,
Scenedesmus obliquus, Synechocystis aquatilis and Chroococcus turgidus, were conducted in
five 1-liter flasks culture set-ups. The species were grown in two conditions of light, 3 k lux
and 6 klux, two temperature conditions, 24-26°C, and 32-36°C and under natural ambient
condition, were BG-11 was used as media and nutrient source to all species grown. The
effects on the growth was dete1mined by biomass monitoring in terms of ash free dry weight
per ml of sample and the actual cell density in number of cells per ml sample. Results showed
that all species have grown successfully for the duration of 8 days culture.
The C. vulgaris, S. obliquus and S. aquatilis were found to obtain the highest cell mass and
density at 6 k lux and 24-36°C or classified in this study as high-light low temperature
condition, while Chroococcus turgidus grew best in 6 k lux, 34-36°C or low-light high
temperature condition. The second phase was the analysis of samples for detection of fatty
acid composition and concentration of the cells in all conditions. It was dete1mined that direct
transterification procedure has given the most number of peaks as shown from the GC
chromatogram. A total of 22 different fatty acids were identified. The predominant PUF A in
all species is a-linolenic acid or C18:3m3. The two green algae, Chlorella vulgaris and
Scenedesmus obliquus were found to obtain the highest yield equivalent at 3 klux, 34-65°C
and 6 k lux at 24-26°C, respectively. Likewise, among the best growing conditions that
enhanced the C18:3ro3 production, Chlorella vulgaris and Scenedesmus obliquus consistently
got the highest concentration and yields of these important nutrients. |
| Year | 2001 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BP-01-09 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Rakshit, Sudip K.; |
| Examination Committee(s) | Jindal, V. K.;Athapol Noomhorm; |
| Scholarship Donor(s) | Royal Thailand Government (RTG)
H.M. Queen's Scholarship Program ; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2001 |