Early stage development of napolean wrasse (Cheilinus undulatus) larvae | |
| Author | Ateng Supriatna |
| Call Number | AIT Thesis no.AQ-03-14 |
| Subject(s) | Fishes -- arvae Wrasses Fishes -- Food |
| Note | A thesis submitted in partial fulfillment of requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-03-14 |
| Abstract | The study on development of napoleon wrasse, Cheilinus undulatus, larvae was conducted in Gondol Research Institute for Mariculture Bali Indonesia from January to April 2003. The purpose of the study was to understand the development of Napoleon wrasse eggs, to evaluate the early life history of napoleon wrasse larvae, to determine the food preference and daily feeding amount, mortality and to assess the effect of prey increment of napoleon wrasse larvae under different color tanks. The result showed that napoleon wrasse spawned in the morning following the lunar cycle. The eggs were transparent, adhesive and spherical in shape with mean diameter of 654 ±12.31 microns. The eggs hatched out between 14 to 16 h after spawning in incubation tank with temperature between 27 to 29°C and salinity of 33%0. The newly hatched (0 hour after hatching) larvae of napoleon wrasse floated directly on the surface of the water. Eyes, alimeata1y canal and pectoral fins of newly hatched larvae appeared only partly developed. Within 1 to 5 hours after hatching, the volume of the yolk and the total length increased rapidly (the yolk from 623 .46 x 104 mm3 to 920.37 x 10-4 mm3 , and the total length from 1.74 ± 0.02 mm to 2.22 ± 0.02 mm). The osteological development from day 1 to day 6 indicated poor development of bony element. At that stage, all of the osteological structures such as caudal fin, notochord, and mouth cavity were dominated by cartilage. The mean total length of larvae measured at hatch (day-1) was 2.47 ± 0.08 mm (N=12) and 2.51± 0.03 mm, respectively on fed and unfed larvae. The larvae continued to grow till day 3 reaching 2.56 ± 0.08 mm and 2.58 ± 0.05 mm in total length. During the early stage (1 to 6 day), the napoleon wrasse larvae had negative growth from 2.47 ± 0.08 mm to 2.24 ± 0.07 mm. A newly hatched napoleon wrasse larvae possessed the yolk and oil globule. The average yolk volume of larvae at hatch (0 h time after hatching) was 623.46 ± 92.761 x 10-4 mm3 and the oil globule was 9.04 x 10-4 mm3 . The larvae had two phases of yolk and oil globule resorption (fast and slow). On delayed feeding, the mortality pattern of the larvae was not significantly different among treatments (P>0.05). All the larvae died at day 7. On daily feeding observation indicated that the larvae had the poor ability to ingest food. The larvae started to ingest food on day 4 after hatching. And the larvae did not consume the ss type rotifers. They improved their ability to capture food on the colored tanks. The larvae culture in the colored tanks such as black, blue and orange increased their ingestion on oyster eggs and/or trocopores. Results in this study indicate that oyster eggs and/or trocopores is the preferred choice of live food compared to rotifers. Larvae not only consume but also have the ability to digest oyster eggs/trocopores. Moreover, choice of tank color and lighting seems to influence the ingestion rate and on subsequent survival. This study advances the survival rate to 7 days compared to the previous 3 days. |
| Year | 2003 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-03-14 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Bart, Amrit; |
| Examination Committee(s) | Amararatne Yakupitiyage ;Mair, Graham C; |
| Scholarship Donor(s) | PAATP, ADB Loan No. 1526-INO; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2003 |