Evaluation of the effect of dietary vitamin C, E and Zinc supplementation on reproductive performance of Nile tilapia (Oreochromis niloticus L.) | |
| Author | Gammanpila, Menake |
| Call Number | AIT Thesis no.AQ-03-36 |
| Subject(s) | Nile tilapia Zinc Vitamin E in animal nutrition Vitamin C in animal nutrition |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. AQ-03-36 |
| Abstract | A 3x2 factorial experiment was conducted to evaluate the role of dietary vitamin C (ascorbic acid) and vitamin E (cx:-tocopherol) and Zinc supplementation on Oreochromis niloticus growth, survival and reproduction in hapa based system installed in eaiihern ponds for 5 months. The triplicate groups consisting 30 fish were fed three different levels of vitamin C (0, 50 and 1250 mg/kg diet), two levels of vitamin E (0, 600 mg/kg diet). An additional treatment that supplemented Zinc at a rate of 120 mg/kg with higher vitamin C and E levels also included in the experiment. The supplementation of vitamin E was not affected on brood fish growth and survival. Vitamin C deficient fish exhibited significantly lower daily weight gain and percentage weight gain compared to the supplementation of higher vitamin C in the diet (P<0.05). The 3x2 factorial analysis of variance indicates that there were no significant effect of supplementation of vitamin C or E on spawning efficiency, total seed production and no of eggs/spawner. However, the spawning percentage, total no of spawns and total seed production in hapa (80.0±28.28, 26±6 and 10,991±1334) were higher in fish fed the dietary Zinc supplemented diet. The brood fish fed diets supplemented of with 1250 mg/kg vitamin C and 600 mg/kg vitamin E had a higher mean clutch size and relative fecundity (626±80 and 7462±588). However, there were no significant differences between 50 mg/kg supplemented level and 1250 mg/kg level of vitamin C on mean clutch size and relative fecundity. The feiiilization rate, egg hatchability and larval survival were 76.19±5.58 and 88.89±4.68, 19.13±8.56 and 45.92±6.33, 57.74±15.02 and 81.09±2.93 for females fed on diets without supplementing vitamins and diet supplemented with higher levels of vitamin C and E, respectively. There was a high correlation between gonadal ascorbate content and a-tocopherol concentration in diets. Fish fed diet without ascorbic acid had reduced spe1m viability and sperm motility compared with supplementation of 1250 mg vitamin C/kg in the diet. No significant positive effects were observed on egg quality or sperm quality of O.niloticus fed higher zinc level in the diet. Present findings suggest that the dietary vitamin C supplementation of 50 mg/kg diet and 120 mg/kg Zinc in the diet support maximum egg production, egg quality and sperm quality. Higher vitamin C levels (1250 mg/kg) in the diet may not be necessary to enhance seed production. |
| Year | 2003 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. AQ-03-36 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Aquaculture and Aquatic Resources Management (AQ) |
| Chairperson(s) | Yakupitiyage, Amararatne; |
| Examination Committee(s) | Bart, Amrit ;Mair, Graham C. ; |
| Scholarship Donor(s) | Government of Netherlands; |
| Degree | Thesis (M.Sc.) - Asian Institute of Technology, 2003 |