Production of specific oligosaccharides from cassava starch by the use of industrial amylase and controlled yeast fermentation | |
| Author | Sivarooban, Theivendran |
| Call Number | AIT Thesis no.BP-02-02 |
| Subject(s) | Oligosaccharides Starch--Synthesis |
| Note | A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, School of Environment, Resources and Development |
| Publisher | Asian Institute of Technology |
| Series Statement | Thesis ; no. BP-02-02 |
| Abstract | Starch is an essential compound for the production of maltooligosaccharides. In case of fructose and glucose production complete hydrolysis is wanted, \Vhereas for ol igosaccharide production the process is designed for reproducible but incomplete hydrol ysis. incomplete hydrolysis of starch leads to the formation of low molecular weight products such as dextrins and maltooligosaccharicles. With the scope of producing high yield of specific maltooligosacchari cles from cassava starch, various starches were analysed for the incomplete hydrolysis by industrial enzymes. These studies were carried out at different temperature with different incubation periods. Wheat starch was hydrolysed at the same condition as cassava starch for the comparative study on temperature effect of a - amylolysis of starch. Samples were taken at defined conditions (Enzyme dosage. Temperature. Incubation time) from all experiments those treated by enzymes and the hydro lytic products were analysed by chemical analysis as well as high performance liquid chromatography (HPLC). Termamyl is an ideal thermostable enzyme for the starch liquefying process in the industrial applications. Multiple attack of this enzyme initiated with the binding of the starch granules. In different temperature conditions Termarnyl acting on starch granules resulting different oligosaccharide profiles for cassava starch hydrolysis. It may be due to the action pattern of the enzyme. Increasing temperature always led to the high range production of maltose (G2) in the end products. Moreover observation reveals that the production of maltose is not only from the further degradation of parent polysaccharide chain but also from the degradation of higher oligosaccharides mainly from pentaose (GS). At 90°C Termamyl preferentially cuts the dimer molecules from the linear chain. Even though the yield of G4 and GS at 90°C was around three times higher than the 5S°C hydrolysis. The results indicate the possibility of high yield production of specific oligosaccharides can be achieved by higher temperature (90°C) treatment ·with control incubation period (6h). Product inhibition was observed in the enzymatic hydrolysis of cassava starch. The removal of end product and dilutions enhance the activity of Termamyl. Enzyme retention observed in the both, in residual starch and hydrolysate, although the remainirig activity of the enzyme was higher in the residual starch at SS°C. Enzyme microbial system for the production of oligosaccharides was analysed for high yield performance. It was observed that the selective efficient removal of lower sccharides from the mixture of hydrolysate by yeast cells could be extended by optimized conditions. The concentration of yeast cell and the concentration of hydrolysate are the major facto rs in the reaction mixture. Additionally, the stage of the yeast cell and availability of Nitrogen sources in the medium also play a role in the selective removal process. Maltooligosaccharides (mainly contain GS and G4) from cassava starch was tested .as functional food for the beneficial intestinal bacteria_, Lactobacillus breviS. It was comparable with the commercially available fructooligosaccharides for the growth of the intestinal organisms. |
| Year | 2002 |
| Corresponding Series Added Entry | Asian Institute of Technology. Thesis ; no. BP-02-02 |
| Type | Thesis |
| School | School of Environment, Resources, and Development (SERD) |
| Department | Department of Food, Agriculture and Natural Resources (Former title: Department of Food Agriculture, and BioResources (DFAB)) |
| Academic Program/FoS | Bioprocess Technology (BP) |
| Chairperson(s) | Stevens, Willem F; |
| Examination Committee(s) | Athapol Noomhorm;Suwalee Chandrhachang;Suwabun Chirachanchai; |
| Scholarship Donor(s) | The Royal Netherlands Government; |
| Degree | Thesis (M. Sc.) - Asian Institute of Technology, 2002 |